ACOT13/THEM2 Recombinant Protein | ACOT13 recombinant protein

ACOT13/THEM2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121873

• Gene Names: ACOT13; HT012; THEM2; PNAS-27
• Purity: >85% as determined by SDS-PAGE
• Synonyms: ACOT13/THEM2; ACOT13/THEM2 Protein; Human; Recombinant (His Tag); Human THEM2/ACOT13 Protein (His Tag); ACOT13 Protein; HT012 Protein; PNAS-27 Protein; THEM2 Protein; acyl-CoA thioesterase 13; ACOT13 recombinant protein

• Host: E Coli
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 100mM Arg, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asn140

• Species: Human
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human THEM2 (NP_060943.1)(Met1-Asn140) was expressed with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for ACOT13 recombinant protein

Background: The metabolites of fatty acyl-Coenzyme A (CoA) and metabolic enzymes contribute to lipid biosynthesis, signal transduction, and gene transcription. Members of the acyl-CoA thioesterase (Acot) gene family hydrolyze fatty acyl-CoAs, but their biological functions remain incompletely understood. Thioesterase superfamily member 2 (Them2; synonym Acot13) is enriched in oxidative tissues, associated with mitochondria, and relatively specific for long chain fatty acyl-CoA substrates. Using Them2(-/-) mice, we have demonstrated key roles for Them2 in regulating hepatic glucose and lipid metabolism. Members of the acyl-CoA thioesterase (Acot) gene family catalyze the hydrolysis of fatty acyl-CoAs, but their biological functions remain unknown. Thioesterase superfamily member 2 (Them2; synonym Acot13) is a broadly expressed mitochondria-associated Acot. Them2 was previously identified as an interacting protein of phosphatidylcholine transfer protein (PC-TP).

NCBI and Uniprot Product Information

• NCBI GI #: 8923812
• NCBI GeneID: 55856
• NCBI Accession #: NP_060943.1
• NCBI GenBank Nucleotide #: NM_018473.3
• UniProt Accession #: Q9NPJ3
• NCBI Official Full Name: acyl-coenzyme A thioesterase 13 isoform 1
• NCBI Official Synonym Full Names: acyl-CoA thioesterase 13
• NCBI Official Symbol: ACOT13
• NCBI Official Synonym Symbols: HT012; THEM2; PNAS-27
• NCBI Protein Information: acyl-coenzyme A thioesterase 13
• UniProt Protein Name: Acyl-coenzyme A thioesterase 13
• Protein Family: Acyl-coenzyme A thioesterase
• UniProt Gene Name: ACOT13
• UniProt Synonym Gene Names: THEM2; Acyl-CoA thioesterase 13
• UniProt Entry Name: ACO13_HUMAN

NCBI Description

This gene encodes a member of the thioesterase superfamily. In humans, the protein co-localizes with microtubules and is essential for sustained cell proliferation. The orthologous mouse protein forms a homotetramer and is associated with mitochondria. The mouse protein functions as a medium- and long-chain acyl-CoA thioesterase. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2009]

Uniprot Description

THEM2: Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Has acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates. Can also hydrolyze 3- hydroxyphenylacetyl-CoA and 3,4-dihydrohyphenylacetyl-CoA (in vitro). Belongs to the thioesterase PaaI family.

Protein type: EC 3.1.2.-

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: mitochondrion; spindle; nucleus; cytosol

Molecular Function: acyl-CoA hydrolase activity

Biological Process: metabolic process; protein homotetramerization