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Immunohistochemistry (IHC) (Anti-ACOT13 / THEM2 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Rabbit THEM2 / ACOT13 Polyclonal Antibody | anti-THEM2 antibody

Rabbit Polyclonal to Human THEM2 / ACOT13

Gene Names
ACOT13; HT012; THEM2; PNAS-27
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Immunoaffinity Purified
Synonyms
THEM2 / ACOT13; Polyclonal Antibody; Rabbit Polyclonal to Human THEM2 / ACOT13; Anti-THEM2 / ACOT13 Antibody (C-Terminus) IHC-plus; ACOT13; Acyl-CoA thioesterase 13; HT012; PNAS-27; Hypothalamus protein HT012; THEM2; Human THEM2; anti-THEM2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Human THEM2
Purity/Purification
Immunoaffinity Purified
Form/Format
PBS, 0.02% sodium azide
Concentration
1 mg/ml (varies by lot)
Sequence Length
140
Applicable Applications for anti-THEM2 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes
ELISA, IHC-P (5 ug/ml), WB (1 - 2 ug/ml)
Usage: THEM2 antibody can be used for detection of THEM2 by Western blot at 1-2 ug/ml.
Target Species
Human
Immunogen Description
A 17 amino acid peptide near the carboxy terminus of human THEM2.
Immunogen Type
Synthetic peptide
Immunogen
THEM2 / ACOT13 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human THEM2.
Antigen Modification
C-Terminus
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-ACOT13 / THEM2 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC) (Anti-ACOT13 / THEM2 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-ACOT13 / THEM2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC) (Anti-ACOT13 / THEM2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of THEM2 in human liver tissue with THEM2 antibody at 20 ug/ml.)

Immunofluorescence (IF) (Immunofluorescence of THEM2 in human liver tissue with THEM2 antibody at 20 ug/ml.)

Western Blot (WB)

(Western blot of THEM2 in HepG2 cell lysate with THEM2 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.)

Western Blot (WB) (Western blot of THEM2 in HepG2 cell lysate with THEM2 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
12,366 Da
NCBI Official Full Name
acyl-coenzyme A thioesterase 13 isoform 1
NCBI Official Synonym Full Names
acyl-CoA thioesterase 13
NCBI Official Symbol
ACOT13
NCBI Official Synonym Symbols
HT012; THEM2; PNAS-27
NCBI Protein Information
acyl-coenzyme A thioesterase 13; hypothalamus protein HT012; thioesterase superfamily member 2
UniProt Protein Name
Acyl-coenzyme A thioesterase 13
UniProt Gene Name
ACOT13
UniProt Synonym Gene Names
THEM2; Acyl-CoA thioesterase 13
UniProt Entry Name
ACO13_HUMAN

NCBI Description

This gene encodes a member of the thioesterase superfamily. In humans, the protein co-localizes with microtubules and is essential for sustained cell proliferation. The orthologous mouse protein forms a homotetramer and is associated with mitochondria. The mouse protein functions as a medium- and long-chain acyl-CoA thioesterase. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2009]

Uniprot Description

THEM2: Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Has acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates. Can also hydrolyze 3- hydroxyphenylacetyl-CoA and 3,4-dihydrohyphenylacetyl-CoA (in vitro). Belongs to the thioesterase PaaI family.

Protein type: EC 3.1.2.-

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: mitochondrion; spindle; cytosol; nucleus

Molecular Function: acyl-CoA hydrolase activity

Biological Process: metabolic process; protein homotetramerization

Research Articles on THEM2

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Product Notes

The THEM2 acot13 (Catalog #AAA243168) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Human THEM2 / ACOT13 reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's THEM2 / ACOT13 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA). ELISA, IHC-P (5 ug/ml), WB (1 - 2 ug/ml) Usage: THEM2 antibody can be used for detection of THEM2 by Western blot at 1-2 ug/ml. Researchers should empirically determine the suitability of the THEM2 acot13 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "THEM2 / ACOT13, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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