Rabbit THEM2 / ACOT13 Polyclonal Antibody | anti-THEM2 antibody

Rabbit Polyclonal (IgG) to Human THEM2 / ACOT13

Cat. Num. AAA244503

• Gene Names: ACOT13; HT012; THEM2; PNAS-27
• Reactivity: Chimpanzee, Human, Orangutan, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Protein A Purified
• Synonyms: THEM2 / ACOT13; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human THEM2 / ACOT13; Anti-THEM2 / ACOT13 Antibody (aa100-150) IHC-plus; ACOT13; Acyl-CoA thioesterase 13; HT012; PNAS-27; Hypothalamus protein HT012; THEM2; Human THEM2; anti-THEM2 antibody

• Host: Rabbit
• Reactivity: Chimpanzee, Human, Orangutan, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human ACOT13 / THEM2
• Purity/Purification: Protein A Purified
• Form/Format: PBS, 0.05% BSA, 0.05% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 140

• Applicable Applications for anti-THEM2 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (10 ug/ml), WB (0.05 - 0.1 ug/ml)
• Target Species: Human
• Immunogen Description: Amino acids 100-150 of human THEM2.
• Immunogen Type: Synthetic peptide
• Immunogen: THEM2 / ACOT13 antibody was raised against amino acids 100-150 of human THEM2.
• Antigen Modification: aa100-150
• Preparation and Storage: Store at 4 degree C for short term applications. For long term storage, aliquot and store at -20 degree C.

Immunohistochemistry (IHC)

(Anti-THEM2 / ACOT13 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.)

Western Blot (WB)

(Western blot of THEM2 in 293 cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) RAW cell lysate using at 0.05 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 8923812
• NCBI GeneID: 55856
• NCBI Accession #: NP_060943.1
• NCBI GenBank Nucleotide #: NM_018473.3
• UniProt Accession #: Q9NPJ3; O95549; F5H2L4
• Molecular Weight: 12,366 Da
• NCBI Official Full Name: acyl-coenzyme A thioesterase 13 isoform 1
• NCBI Official Synonym Full Names: acyl-CoA thioesterase 13
• NCBI Official Symbol: ACOT13
• NCBI Official Synonym Symbols: HT012; THEM2; PNAS-27
• NCBI Protein Information: acyl-coenzyme A thioesterase 13; hypothalamus protein HT012; thioesterase superfamily member 2
• UniProt Protein Name: Acyl-coenzyme A thioesterase 13
• UniProt Gene Name: ACOT13
• UniProt Synonym Gene Names: THEM2; Acyl-CoA thioesterase 13
• UniProt Entry Name: ACO13_HUMAN

NCBI Description

This gene encodes a member of the thioesterase superfamily. In humans, the protein co-localizes with microtubules and is essential for sustained cell proliferation. The orthologous mouse protein forms a homotetramer and is associated with mitochondria. The mouse protein functions as a medium- and long-chain acyl-CoA thioesterase. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2009]

Uniprot Description

THEM2: Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Has acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates. Can also hydrolyze 3- hydroxyphenylacetyl-CoA and 3,4-dihydrohyphenylacetyl-CoA (in vitro). Belongs to the thioesterase PaaI family.

Protein type: EC 3.1.2.-

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: mitochondrion; spindle; nucleus; cytosol

Molecular Function: acyl-CoA hydrolase activity

Biological Process: metabolic process; protein homotetramerization