Rabbit THEM2 Polyclonal Antibody | anti-ACOT13 antibody

THEM2 Antibody

Cat. Num. AAA150103

• Gene Names: ACOT13; HT012; THEM2; PNAS-27
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: THEM2 Antibody is affinity chromatography purified via peptide column.
• Synonyms: THEM2; Polyclonal Antibody; THEM2 Antibody; HT012; PNAS-27; Acyl-coenzyme A thioesterase 13; Thioesterase superfamily member 2; Acyl-CoA thioesterase 13; acyl-CoA thioesterase 13; anti-ACOT13 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: THEM2 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 140

• Applicable Applications for anti-ACOT13 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: THEM2 antibody can be used for detection of THEM2 by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: THEM2 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human THEM2.
• Buffer: THEM2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: THEM2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of THEM2 in HepG2 cell lysate with THEM2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)

Immunohistochemistry (IHC)

(Immunohistochemistry of THEM2 in human liver tissue with THEM2 antibody at 2.5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of THEM2 in human liver tissue with THEM2 antibody at 20 μg/mL.)

Related Product Information for anti-ACOT13 antibody

THEM2 Antibody: THEM2, also known as ACOT13, belongs to the hotdog-fold superfamily and possesses thioesterase activity, with greater activity observed with longer chain acyl-CoAs such as myristoyl- and palmitoyl-CoA. THEM2 is highly expressed in several tissues such as heart, kidney, liver and brain and has been shown to be co-localized with beta-tubulin on microtubules. THEM2 interacts with StarD, a protein that plays a key role in fatty acid metabolism, and the addition of StarD to THEM2 increased its catalytic activity, suggesting that THEM2 plays a significant role in the metabolism of fatty acids. At least two isoforms of THEM2 are known to exist.

NCBI and Uniprot Product Information

• NCBI GI #: 8923812
• NCBI GeneID: 55856
• NCBI Accession #: NP_060943
• NCBI GenBank Nucleotide #: NM_018473.3
• UniProt Accession #: Q9NPJ3; O95549; F5H2L4
• Molecular Weight: 12,366 Da
• NCBI Official Full Name: acyl-coenzyme A thioesterase 13 isoform 1
• NCBI Official Synonym Full Names: acyl-CoA thioesterase 13
• NCBI Official Symbol: ACOT13
• NCBI Official Synonym Symbols: HT012; THEM2; PNAS-27
• NCBI Protein Information: acyl-coenzyme A thioesterase 13; hypothalamus protein HT012; thioesterase superfamily member 2
• UniProt Protein Name: Acyl-coenzyme A thioesterase 13
• Protein Family: Acyl-coenzyme A thioesterase
• UniProt Gene Name: ACOT13
• UniProt Synonym Gene Names: THEM2; Acyl-CoA thioesterase 13
• UniProt Entry Name: ACO13_HUMAN

NCBI Description

This gene encodes a member of the thioesterase superfamily. In humans, the protein co-localizes with microtubules and is essential for sustained cell proliferation. The orthologous mouse protein forms a homotetramer and is associated with mitochondria. The mouse protein functions as a medium- and long-chain acyl-CoA thioesterase. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2009]

Uniprot Description

THEM2: Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Has acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates. Can also hydrolyze 3- hydroxyphenylacetyl-CoA and 3,4-dihydrohyphenylacetyl-CoA (in vitro). Belongs to the thioesterase PaaI family.

Protein type: EC 3.1.2.-

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: mitochondrion; spindle; cytosol; nucleus

Molecular Function: acyl-CoA hydrolase activity

Biological Process: metabolic process; protein homotetramerization