Rabbit ALDH3A2 Polyclonal Antibody | anti-ALDH3A2 antibody

Anti-ALDH3A2 Antibody, Rabbit Polyclonal

Cat. Num. AAA8102368

• Gene Names: Aldh3a2; Ahd3; Ahd-3; Aldh4; FALDH; Ahd-3r; Ahd3-r; Aldh4-r; AI194803
• Reactivity: Mouse; Predicted to React with: Human, Rat (Species predicted to react based on 100% sequence homology)
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: ALDH3A2; Polyclonal Antibody; Anti-ALDH3A2 Antibody; Rabbit Polyclonal; ALDH3A2 Antibody; Rabbit PAb; Antigen Affinity Purified; aldehyde dehydrogenase 3 family; member A2; Anti-Ahd-3 Antibody; Anti-Ahd-3r Antibody; Anti-Ahd3 Antibody; Anti-Ahd3-r Antibody; Anti-AI194803 Antibody; Anti-Aldh4 Antibody; Anti-Aldh4-r Antibody; Anti-FALDH Antibody; anti-ALDH3A2 antibody

• Host: Rabbit
• Reactivity: Mouse; Predicted to React with: Human, Rat (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse ALDH3A2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-ALDH3A2 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; IHC-P: 1:2000-1:10,000; IP: 0.2-1uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Mouse ALDH3A2
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Mouse ALDH3A2, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-ALDH3A2 rabbit polyclonal antibody at 1:500 dilutionLane A: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:55 kDaObserved band size:54 kDa)

Immunohistochemistry (IHC)

(Immunochemical staining of mouse Aldh3a2 in mouse liver with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)

Immunoprecipitation (IP)

(Aldh3a2 was immunoprecipitated using:Lane A:0.5 mg A549 Whole Cell Lysate0.5 uL anti-Aldh3a2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Aldh3a2 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:500 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 53 kDaObserved band size: 53 kDa)

Related Product Information for anti-ALDH3A2 antibody

Sjogren-Larsson syndrome (SLS) is a rare type of congenital ichthyosis with neurological problems and intellectual disability. Homozygous mutations in ALDH3A2 gene are known to be responsible for this syndrome. An ALDH3A2 gene mutation results in dysfunction of the microsomal enzyme fatty aldehyde dehydrogenase and impaired metabolism and accumulation of leukotriene B4, which is a key molecule and a pro-inflammatory mediator in developing allergic diseases, especially asthma. An increased level of leukotriene B4 has been reported in SLS patients.

NCBI and Uniprot Product Information

• NCBI GI #: 75677435
• NCBI GeneID: 11671
• NCBI Accession #: NP_031463.2
• NCBI GenBank Nucleotide #: NM_007437.4
• UniProt Accession #: P47740; Q99L64
• Molecular Weight: 53,971 Da
• NCBI Official Full Name: fatty aldehyde dehydrogenase
• NCBI Official Synonym Full Names: aldehyde dehydrogenase family 3, subfamily A2
• NCBI Official Symbol: Aldh3a2
• NCBI Official Synonym Symbols: Ahd3; Ahd-3; Aldh4; FALDH; Ahd-3r; Ahd3-r; Aldh4-r; AI194803
• NCBI Protein Information: fatty aldehyde dehydrogenase; aldehyde dehydrogenase 3; aldehyde dehydrogenase family 3 member A2; alcohol dehydrogenase family 3, subfamily A2; aldehyde dehydrogenase 4, liver microsomal (class 3) regulator
• UniProt Protein Name: Fatty aldehyde dehydrogenase
• Protein Family: Fatty aldehyde dehydrogenase
• UniProt Gene Name: Aldh3a2
• UniProt Synonym Gene Names: Ahd-3; Ahd3; Aldh3; Aldh4
• UniProt Entry Name: AL3A2_MOUSE

Uniprot Description

ALDH3A2: Catalyzes the oxidation of long-chain aliphatic aldehydes to fatty acids. Active on a variety of saturated and unsaturated aliphatic aldehydes between 6 and 24 carbons in length. Responsible for conversion of the sphingosine 1-phosphate (S1P) degradation product hexadecenal to hexadecenoic acid. Defects in ALDH3A2 are the cause of Sjoegren-Larsson syndrome (SLS). SLS is an autosomal recessive neurocutaneous disorder characterized by a combination of severe mental retardation, spastic di- or tetraplegia and congenital ichthyosis (increased keratinization). Ichthyosis is usually evident at birth, neurologic symptoms appear in the first or second year of life. Most patients have an IQ of less than 60. Additional clinical features include glistening white spots on the retina, seizures, short stature and speech defects. Belongs to the aldehyde dehydrogenase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - histidine; Carbohydrate Metabolism - ascorbate and aldarate; Carbohydrate Metabolism - propanoate; EC 1.2.1.3; Carbohydrate Metabolism - pyruvate; Oxidoreductase; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Membrane protein, integral; Amino Acid Metabolism - tryptophan; Mitochondrial; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Lipid Metabolism - glycerolipid; Lipid Metabolism - fatty acid; Amino Acid Metabolism - lysine degradation; Amino Acid Metabolism - arginine and proline; Secondary Metabolites Metabolism - limonene and pinene degradation; Other Amino Acids Metabolism - beta-alanine; Carbohydrate Metabolism - butanoate

Cellular Component: mitochondrion; membrane; intracellular membrane-bound organelle; endoplasmic reticulum; mitochondrial inner membrane; integral to membrane; peroxisome; nucleus; cytosol

Molecular Function: long-chain-alcohol oxidase activity; aldehyde dehydrogenase (NAD) activity; aldehyde dehydrogenase [NAD(P)+] activity; 3-chloroallyl aldehyde dehydrogenase activity; long-chain-aldehyde dehydrogenase activity; oxidoreductase activity; oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor

Biological Process: phytol metabolic process; response to reactive oxygen species; epidermis development; central nervous system development; metabolic process; formaldehyde metabolic process; aldehyde metabolic process; sesquiterpenoid metabolic process; peripheral nervous system development