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SDS-Page

IL-33 active protein

IL-33, 112-270aa, Human, E Coli (Bioactivity Validated)

Gene Names
IL33; DVS27; IL1F11; NF-HEV; NFEHEV; C9orf26
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
IL-33; 112-270aa; Human; E Coli (Bioactivity Validated); Interleukin 33; DVS27; NF-HEV; NKHEV; C9orf26; Interleukin 33 CHROMOSOME 9 OPEN READING FRAME 26; DKFZp586H0523; DVS27 related protein; IL 1F11; IL 33; IL1F11; Interleukin 1 family member 11; INTERLEuKIN 33 NFHEV; Interleukin 33 precursor; Interleukin33; NF HEV; NFHEV; Nuclear factor from high endothelial venules; RP11 575C20.2; il33; IL-33 active protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
In 20mM Tris 8.0
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MSITGISPIT EYLASLSTYN DQSITFALED ESYEIYVEDL KKDEKKDKVL LSYYESQHPS NESGDGVDGK MLMVTLSPTK DFWLHANNKE HSVELHKCEK PLPDQAFFVL HNMHSNCVSF ECKTDPGVFI GVKDNHLALI KVDSSENLCT ENILFKLSET
Sequence Length
270
Applicable Applications for IL-33 active protein
SDS-PAGE
Antigen Species
Human
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

SDS-Page

SDS-Page
Related Product Information for IL-33 active protein
Interleukin 33 (IL-33) is a 32kDa proinflammatory cytokine that may also regulate gene transcription in producer cells. IL-33 is structurally related to IL-1, which induces helper T cells to produce type 2 cytokines and acts through the receptor IL1RL-1 (IL1 receptor-like-1), which is known also as ST2. Binding of IL-33 to this receptor activates NF-kappa-B and MAP kinases and induces in vitro Th2 cells to produce cytokines. In vivo, IL-33 induces expression of IL-4, IL-5, IL-13 and leads to severe pathological changes in mucosal organs and in vitro, it can be divided to N-terminal fragment of 12kDa and C-terminal fragment of 18kDa by cleavage of caspase-1. Recombinant human IL-33 (C-terminal fragment) was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for IL-33 active protein
References
Schmitz., et al. (2005) immunity 23 (5):479-90; Baekkevold, E.S., et al. (2003) Am. J.Pathol. 163 (1):69-90;

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
18.1Da (160aa), confirmed by MALDI-TOF.
NCBI Official Full Name
interleukin-33 isoform a
NCBI Official Synonym Full Names
interleukin 33
NCBI Official Symbol
IL33
NCBI Official Synonym Symbols
DVS27; IL1F11; NF-HEV; NFEHEV; C9orf26
NCBI Protein Information
interleukin-33
UniProt Protein Name
Interleukin-33
UniProt Gene Name
IL33
UniProt Synonym Gene Names
C9orf26; IL1F11; NFHEV; IL-33; IL-1F11; NF-HEV

NCBI Description

The protein encoded by this gene is a cytokine that binds to the IL1RL1/ST2 receptor. The encoded protein is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

Uniprot Description

Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells (PubMed:16286016). Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury (PubMed:17853410, PubMed:18836528).

Research Articles on IL-33

Similar Products

Product Notes

The IL-33 il33 (Catalog #AAA203099) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's IL-33 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the IL-33 il33 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MSITGISPIT EYLASLSTYN DQSITFALED ESYEIYVEDL KKDEKKDKVL LSYYESQHPS NESGDGVDGK MLMVTLSPTK DFWLHANNKE HSVELHKCEK PLPDQAFFVL HNMHSNCVSF ECKTDPGVFI GVKDNHLALI KVDSSENLCT ENILFKLSET. It is sometimes possible for the material contained within the vial of "IL-33, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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