Human Metalloproteinase inhibitor 4 ELISA Kit | TIMP-4 elisa kit

Human Metalloproteinase inhibitor 4 ELISA Kit

Cat. Num. AAA764570

• Reactivity: Human
• Synonyms: Metalloproteinase inhibitor 4; Human Metalloproteinase inhibitor 4 ELISA Kit; TIMP-4/TIMP metallopeptidase inhibitor 4/tissue inhibitor of metalloproteinase 4; TIMP-4 elisa kit

• Reactivity: Human
• Sequence Length: 224

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 78-5000pg/ml
• Sensitivity: 46.875pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for TIMP-4 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-TIMP-4 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-TIMP-4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TIMP-4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TIMP-4 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4507515
• NCBI GeneID: 7079
• NCBI Accession #: NP_003247.1
• NCBI GenBank Nucleotide #: NM_003256.3
• Molecular Weight: 25,503 Da
• NCBI Official Full Name: metalloproteinase inhibitor 4
• NCBI Official Synonym Full Names: TIMP metallopeptidase inhibitor 4
• NCBI Official Symbol: TIMP4
• NCBI Protein Information: metalloproteinase inhibitor 4
• UniProt Protein Name: Metalloproteinase inhibitor 4
• Protein Family: Metalloproteinase inhibitor
• UniProt Gene Name: TIMP4
• UniProt Synonym Gene Names: TIMP-4
• UniProt Entry Name: TIMP4_HUMAN

NCBI Description

This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. The secreted, netrin domain-containing protein encoded by this gene is involved in regulation of platelet aggregation and recruitment and may play role in hormonal regulation and endometrial tissue remodeling. [provided by RefSeq, Jul 2008]

Uniprot Description

TIMP4: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP- 9. Belongs to the protease inhibitor I35 (TIMP) family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3p25

Cellular Component: extracellular space; proteinaceous extracellular matrix; sarcomere

Molecular Function: metal ion binding; metalloendopeptidase inhibitor activity; protease binding

Biological Process: central nervous system development; negative regulation of membrane protein ectodomain proteolysis; negative regulation of metalloenzyme activity; Notch signaling pathway; ovulation cycle; response to cytokine stimulus; response to drug; response to lipopolysaccharide; response to organic substance; response to peptide hormone stimulus