Mouse TIMP4 ELISA Kit | TIMP4 elisa kit

Mouse TIMP4 ELISA Kit

Cat. Num. AAA8244561

• Reactivity: Mouse
• Applications: ELISA
• Synonyms: TIMP4; Mouse TIMP4 ELISA Kit; Metalloproteinase inhibitor 4; Tissue inhibitor of metalloproteinases 4; TIMP-4; TIMP4 elisa kit

• Reactivity: Mouse
• Specificity: The Mouse TIMP4 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse TIMP4 proteins within the range of 78 pg/ml - 5000 pg/ml.
• Sequence Length: 224

• Applicable Applications for TIMP4 elisa kit: Sandwich ELISA (EIA)
• Samples: Cell Culture Supernatants, Serum, Plasma.
• Sensitivity: 10 pg/ml.
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve

Related Product Information for TIMP4 elisa kit

Principle of the Assay||The Mouse TIMP4 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse TIMP4 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse TIMP4 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIMP4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse TIMP4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIMP4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background/Introduction: Metalloproteinase inhibitor 4 is an enzyme that in humans is encoded by the TIMP4 gene. This gene belongs to the tissue inhibitor of metalloproteinases gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. TIMP4 gene is mapped to chromosome 3p25. TIMP4 gene contains 5 exons that span 6 kb of genomic DNA. The gene is expressed as a 1.4-kb transcript abundant in heart and present at low levels in several other tissues. Expressed TIMP4 inhibits MMPs in vitro. What's more, TIMP-4 binds both progelatinase A and the C domain in a similar manner to that of TIMP-2.

NCBI and Uniprot Product Information

• NCBI GI #: 3915135
• NCBI GeneID: 7079
• UniProt Accession #: Q99727; B2R7K6
• Molecular Weight: 25,503 Da
• NCBI Official Full Name: Metalloproteinase inhibitor 4
• NCBI Official Synonym Full Names: TIMP metallopeptidase inhibitor 4
• NCBI Official Symbol: TIMP4
• NCBI Protein Information: metalloproteinase inhibitor 4
• UniProt Protein Name: Metalloproteinase inhibitor 4
• Protein Family: Metalloproteinase inhibitor
• UniProt Gene Name: TIMP4
• UniProt Synonym Gene Names: TIMP-4
• UniProt Entry Name: TIMP4_HUMAN

NCBI Description

This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. The secreted, netrin domain-containing protein encoded by this gene is involved in regulation of platelet aggregation and recruitment and may play role in hormonal regulation and endometrial tissue remodeling. [provided by RefSeq, Jul 2008]

Uniprot Description

TIMP4: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP- 9. Belongs to the protease inhibitor I35 (TIMP) family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3p25

Cellular Component: extracellular space; proteinaceous extracellular matrix; sarcomere

Molecular Function: metal ion binding; metalloendopeptidase inhibitor activity; protease binding

Biological Process: central nervous system development; negative regulation of membrane protein ectodomain proteolysis; negative regulation of metalloenzyme activity; Notch signaling pathway; ovulation cycle; response to cytokine stimulus; response to drug; response to lipopolysaccharide; response to organic substance; response to peptide hormone stimulus