Human TIMP-4 ELISA Kit | TIMP4 elisa kit

Human TIMP-4 PicoKine ELISA Kit

Cat. Num. AAA177395

• Reactivity: Human
• Synonyms: TIMP-4; Human TIMP-4 PicoKine ELISA Kit; Metalloproteinase inhibitor 4; TIMP metallopeptidase inhibitor 4; TIMP 4; Tissue inhibitor of metalloproteinase 4; Tissue inhibitor of metalloproteinases 4; TIMP4 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human TIMP-4. This kit detects recombinant human TIMP-4 in both free and MMP-bound forms.
• Sequence Length: 224

• Samples: Cell culture supernates, serum, plasma (heparin, EDTA) and human milk
• Assay Type: Sandwich
• Detection Range: 78pg/ml-5,000pg/ml
• Sensitivity: < 10pg/ml
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Human TIMP-4 PicoKine ELISA Kit standard curve )

Related Product Information for TIMP4 elisa kit

Principle of the assay: human TIMP-4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TIMP-4 has been precoated onto 96-well plates. Standards(sf21, C30-P224) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TIMP-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TIMP-4 amount of sample captured in plate.
Background: Tissue inhibitor of metalloproteinases 4, also called Metalloproteinase inhibitor 4, is an enzyme that in humans is encoded by the TIMP4 gene. This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. This gene is mapped to 3p25.2. The secreted, netrin domain-containing protein encoded by this gene is involved in regulation of platelet aggregation and recruitment and may play role in hormonal regulation and endometrial tissue remodeling.

NCBI and Uniprot Product Information

• NCBI GI #: 3915135
• NCBI GeneID: 7079
• UniProt Accession #: Q99727; B2R7K6
• Molecular Weight: 25,503 Da
• NCBI Official Full Name: Metalloproteinase inhibitor 4
• NCBI Official Synonym Full Names: TIMP metallopeptidase inhibitor 4
• NCBI Official Symbol: TIMP4
• NCBI Protein Information: metalloproteinase inhibitor 4; TIMP-4; tissue inhibitor of metalloproteinase 4; tissue inhibitor of metalloproteinases 4
• UniProt Protein Name: Metalloproteinase inhibitor 4
• Protein Family: Metalloproteinase inhibitor
• UniProt Gene Name: TIMP4
• UniProt Synonym Gene Names: TIMP-4
• UniProt Entry Name: TIMP4_HUMAN

NCBI Description

This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. The secreted, netrin domain-containing protein encoded by this gene is involved in regulation of platelet aggregation and recruitment and may play role in hormonal regulation and endometrial tissue remodeling. [provided by RefSeq, Jul 2008]

Uniprot Description

TIMP4: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP- 9. Belongs to the protease inhibitor I35 (TIMP) family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 3p25

Cellular Component: extracellular space; proteinaceous extracellular matrix; sarcomere

Molecular Function: metalloendopeptidase inhibitor activity; protease binding; metal ion binding

Biological Process: response to drug; response to peptide hormone stimulus; negative regulation of metalloenzyme activity; central nervous system development; ovulation cycle; response to cytokine stimulus; response to hormone stimulus; response to lipopolysaccharide; negative regulation of membrane protein ectodomain proteolysis