Typical Standard Curve/Testing Data
Human CCR4 NOT transcription complex subunit 6 (CNOT6) ELISA Kit | CNOT6 elisa kit
Human CCR4 NOT transcription complex subunit 6 (CNOT6) ELISA Kit
Reactivity
Human
Synonyms
CCR4 NOT transcription complex subunit 6 (CNOT6); Human CCR4 NOT transcription complex subunit 6 (CNOT6) ELISA Kit; CNOT6 elisa kit
Reactivity
Human
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
50-1000pg/mL
Sensitivity
1.0pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Standard Curve/Testing Data
Typical Standard Curve/Testing Data
Related Product Information for CNOT6 elisa kit
Intended Uses: This CNOT6 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CNOT6. This ELISA kit for research use only!
Principle of the Assay: CNOT6 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CNOT6 antibody and an CNOT6-HRP conjugate. The assay sample and buffer are incubated together with CNOT6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CNOT6 concentration since CNOT6 from samples and CNOT6-HRP conjugate compete for the anti-CNOT6 antibody binding site. Since the number of sites is limited, as more sites are occupied by CNOT6 from the sample, fewer sites are left to bind CNOT6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CNOT6 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: CNOT6 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CNOT6 antibody and an CNOT6-HRP conjugate. The assay sample and buffer are incubated together with CNOT6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CNOT6 concentration since CNOT6 from samples and CNOT6-HRP conjugate compete for the anti-CNOT6 antibody binding site. Since the number of sites is limited, as more sites are occupied by CNOT6 from the sample, fewer sites are left to bind CNOT6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CNOT6 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for CNOT6 elisa kit
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Product Notes
The Human CNOT6 (Catalog #AAA7245614) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7245614 ELISA Kit recognizes Human CNOT6. It is sometimes possible for the material contained within the vial of "CCR4 NOT transcription complex subunit 6 (CNOT6), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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