Typical Testing Data/Standard Curve
Human Chromobox protein homolog 8 (CBX8) ELISA Kit | CBX8 elisa kit
Human Chromobox protein homolog 8 (CBX8) ELISA Kit
Gene Names
CBX8; PC3; RC1
Reactivity
Human
Synonyms
Chromobox protein homolog 8 (CBX8); Human Chromobox protein homolog 8 (CBX8) ELISA Kit; CBX8 elisa kit
Reactivity
Human
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
100-2500pg/mL
Sensitivity
1.0pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Related Product Information for CBX8 elisa kit
Intended Uses: This CBX8 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CBX8. This ELISA kit for research use only!
Principle of the Assay||CBX8 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CBX8 antibody and an CBX8-HRP conjugate. The assay sample and buffer are incubated together with CBX8-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CBX8 concentration since CBX8 from samples and CBX8-HRP conjugate compete for the anti-CBX8 antibody binding site. Since the number of sites is limited, as more sites are occupied by CBX8 from the sample, fewer sites are left to bind CBX8-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CBX8 concentration in each sample is interpolated from this standard curve.
Principle of the Assay||CBX8 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CBX8 antibody and an CBX8-HRP conjugate. The assay sample and buffer are incubated together with CBX8-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CBX8 concentration since CBX8 from samples and CBX8-HRP conjugate compete for the anti-CBX8 antibody binding site. Since the number of sites is limited, as more sites are occupied by CBX8 from the sample, fewer sites are left to bind CBX8-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CBX8 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for CBX8 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43,396 Da
NCBI Official Full Name
chromobox protein homolog 8
NCBI Official Synonym Full Names
chromobox homolog 8
NCBI Official Symbol
CBX8
NCBI Official Synonym Symbols
PC3; RC1
NCBI Protein Information
chromobox protein homolog 8; polycomb 3; rectachrome 1; Pc class 3 homolog; chromobox homolog 8 (Pc class homolog, Drosophila)
UniProt Protein Name
Chromobox protein homolog 8
Protein Family
UniProt Gene Name
CBX8
UniProt Synonym Gene Names
PC3; RC1; Pc3; hPc3
UniProt Entry Name
CBX8_HUMAN
Uniprot Description
CBX8: Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
Protein type: Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 17q25.3
Cellular Component: nucleoplasm; heterochromatin; nuclear chromatin; PcG protein complex; nucleus
Molecular Function: protein binding; single-stranded RNA binding; ubiquitin-protein ligase activity; methylated histone residue binding
Biological Process: transcription, DNA-dependent; histone ubiquitination; negative regulation of transcription from RNA polymerase II promoter
Research Articles on CBX8
Similar Products
Product Notes
The Human CBX8 cbx8 (Catalog #AAA7214672) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7214672 ELISA Kit recognizes Human CBX8. It is sometimes possible for the material contained within the vial of "Chromobox protein homolog 8 (CBX8), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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