Typical Testing Data/Standard Curve
Bovine CCR4-NOT transcription complex subunit 1 (CNOT1) ELISA Kit | CNOT1 elisa kit
Bovine CCR4-NOT transcription complex subunit 1 (CNOT1) ELISA Kit
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Principle of the Assay: CNOT1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-CNOT1 antibody and an CNOT1-HRP conjugate. The assay sample and buffer are incubated together with CNOT1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CNOT1 concentration since CNOT1 from samples and CNOT1-HRP conjugate compete for the anti-CNOT1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CNOT1 from the sample, fewer sites are left to bind CNOT1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CNOT1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
Uniprot Description
NOT1: represses ligand-dependent transcriptional activation by oestrogen receptor (ER) alpha and several RXR heterodimers. Interacts with the ligand-binding domain of estrogen receptor-alpha in a hormone-dependent fashion.
Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor; RNA-binding
Chromosomal Location of Human Ortholog: 16q21
Cellular Component: nucleoplasm; peroxisomal membrane; extracellular space; membrane; cytosol; CCR4-NOT complex
Molecular Function: protein domain specific binding; protein binding; poly(A)-specific ribonuclease activity; retinoic acid receptor binding; estrogen receptor binding; protein complex scaffold
Biological Process: negative regulation of retinoic acid receptor signaling pathway; poly(A) tail shortening; transcription, DNA-dependent; multicellular organismal development; negative regulation of translation; gene expression; negative regulation of transcription from RNA polymerase II promoter; miRNA-mediated gene silencing; negative regulation of estrogen receptor signaling pathway; mRNA catabolic process, deadenylation-dependent decay
Research Articles on CNOT1
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Product Notes
The Bovine CNOT1 cnot1 (Catalog #AAA7228799) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7228799 ELISA Kit recognizes Bovine CNOT1. It is sometimes possible for the material contained within the vial of "CCR4-NOT transcription complex subunit 1 (CNOT1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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