Rabbit anti-Human LSM14A Polyclonal Antibody | anti-LSM14A antibody

LSM14A, CT (LSM14A, C19orf13, FAM61A, RAP55, RAP55A, Protein LSM14 homolog A, Protein FAM61A, Protein SCD6 homolog, Putative alpha-synuclein-binding protein, RNA-associated protein 55A) (Biotin)

Cat. Num. AAA6317474

• Gene Names: LSM14A; RAP55; FAM61A; RAP55A; C19orf13
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: LSM14A; Polyclonal Antibody; CT (LSM14A; C19orf13; FAM61A; RAP55; RAP55A; Protein LSM14 homolog A; Protein FAM61A; Protein SCD6 homolog; Putative alpha-synuclein-binding protein; RNA-associated protein 55A) (Biotin); RNA-associated protein 55A; anti-LSM14A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-LSM14A antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: LSM14A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 310-340 amino acids from the C-terminal region of human LSM14A.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(LSM14A Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the LSM14A antibody detected the LSM14A protein (arrow).)

Immunohistochemistry (IHC)

(LSM14A Antibody (C-term) (AP14733b)immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of LSM14A Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-LSM14A antibody

Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family (see SNRPD2; 601061). Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing.

Product Categories/Family for anti-LSM14A antibody

Antibodies; Synuclein

References

Marnef, A., et al. Int. J. Biochem. Cell Biol. 41(5):977-981(2009). Tanaka, K.J., et al. J. Biol. Chem. 281(52):40096-40106(2006). Olsen, J.V., et al. Cell 127(3):635-648(2006). Yang, W.H., et al. RNA 12(4):547-554(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 166197712
• NCBI GeneID: 26065
• NCBI Accession #: NP_001107565.1; NP_056393.2
• NCBI GenBank Nucleotide #: NM_001114093.1
• UniProt Accession #: Q8ND56; Q76LX7; Q96AR3; Q96K73; Q96SN5; Q9UFR3
• Molecular Weight: 50,530 Da
• NCBI Official Full Name: protein LSM14 homolog A isoform a
• NCBI Official Synonym Full Names: LSM14A, SCD6 homolog A (S. cerevisiae)
• NCBI Official Symbol: LSM14A
• NCBI Official Synonym Symbols: RAP55; FAM61A; RAP55A; C19orf13
• NCBI Protein Information: protein LSM14 homolog A; hRAP55; hRAP55A; alphaSNBP; protein SCD6 homolog; RNA-associated protein 55; RNA-associated protein 55A; putative alpha-synuclein-binding protein; family with sequence similarity 61, member A
• UniProt Protein Name: Protein LSM14 homolog A
• Protein Family: Protein
• UniProt Gene Name: LSM14A
• UniProt Synonym Gene Names: C19orf13; FAM61A; RAP55; RAP55A; AlphaSNBP; hRAP55; hRAP55A
• UniProt Entry Name: LS14A_HUMAN

Uniprot Description

alphaSNBP(A): Essential for formation of P-bodies, cytoplasmic structures that provide storage sites for non-translating mRNAs. Belongs to the LSM14 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Translation; RNA-binding

Chromosomal Location of Human Ortholog: 19q13.11

Cellular Component: intracellular membrane-bound organelle; stress granule; cytoplasm

Molecular Function: single-stranded RNA binding; double-stranded DNA binding; double-stranded RNA binding

Biological Process: regulation of translation; multicellular organismal development; cytoplasmic mRNA processing body assembly