Typical Testing Data/Standard Curve (for reference only)
Human Cyclic AMP dependent transcription factor ATF 5 (ATF5) ELISA Kit | ATF5 elisa kit
Human Cyclic AMP dependent transcription factor ATF 5 (ATF5) ELISA Kit
Gene Names
ATF5; ATFX; HMFN0395
Reactivity
Human
Synonyms
Cyclic AMP dependent transcription factor ATF 5 (ATF5); Human Cyclic AMP dependent transcription factor ATF 5 (ATF5) ELISA Kit; ATF5 elisa kit
Reactivity
Human
Samples
Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 pg/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ATF5 elisa kit
Intended Uses: This ATF4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ATF4. This ELISA kit for research use only!
Principle of the Assay: ATF4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ATF4 antibody and an ATF4-HRP conjugate. The assay sample and buffer are incubated together with ATF4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATF4 concentration since ATF4 from samples and ATF4-HRP conjugate compete for the anti-ATF4 antibody binding site. Since the number of sites is limited, as more sites are occupied by ATF4 from the sample, fewer sites are left to bind ATF4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATF4 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: ATF4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ATF4 antibody and an ATF4-HRP conjugate. The assay sample and buffer are incubated together with ATF4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATF4 concentration since ATF4 from samples and ATF4-HRP conjugate compete for the anti-ATF4 antibody binding site. Since the number of sites is limited, as more sites are occupied by ATF4 from the sample, fewer sites are left to bind ATF4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATF4 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for ATF5 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,674 Da
NCBI Official Full Name
cyclic AMP-dependent transcription factor ATF-5
NCBI Official Synonym Full Names
activating transcription factor 5
NCBI Official Symbol
ATF5
NCBI Official Synonym Symbols
ATFX; HMFN0395
NCBI Protein Information
cyclic AMP-dependent transcription factor ATF-5; transcription factor ATFx; cAMP-dependent transcription factor ATF-5
UniProt Protein Name
Cyclic AMP-dependent transcription factor ATF-5
Protein Family
UniProt Gene Name
ATF5
UniProt Synonym Gene Names
ATFX; cAMP-dependent transcription factor ATF-5
UniProt Entry Name
ATF5_HUMAN
Uniprot Description
ATF-5: Transcriptional activator which binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters and blocks the differentiation of neuroprogenitor cells into neurons. Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4. Belongs to the bZIP family.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 19q13.3
Cellular Component: nucleoplasm; transcription factor complex; cytoplasm
Molecular Function: protein binding; heat shock protein binding; transcription corepressor activity
Biological Process: regulation of transcription from RNA polymerase II promoter; circadian rhythm; transcription, DNA-dependent; olfactory bulb interneuron development; multicellular organism growth; negative regulation of astrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; post-embryonic development; negative regulation of apoptosis
Research Articles on ATF5
Similar Products
Product Notes
The Human ATF5 atf5 (Catalog #AAA7218397) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7218397 ELISA Kit recognizes Human ATF5. It is sometimes possible for the material contained within the vial of "Cyclic AMP dependent transcription factor ATF 5 (ATF5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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