Streptavidin Antibody
Streptavidin-R-PE
Applications
Flow Cytometry, Functional Assay
Synonyms
Streptavidin; Streptavidin-R-PE; Streptavidin/R-PE; anti-Streptavidin antibody
Form/Format
50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA.
Concentration
Volume: 240 ul (varies by lot)
Sequence Length
159
Applicable Applications for anti-Streptavidin antibody
Flow Cytometry (FC/FACS)
Application Notes
1:10 (or use 2 ul of concentrated stock per 5 x 105-cell test)
Performance
When used as a second step reagent in Flow cytometry, optimum resolution between signal and background with biotinylated antibodies was obtained using 20 ul of a 1:10 dilution of product to stain 5 x 105 cells.
Conjugation
PE
Production
Purified streptavidin was covalently conjugated to R-Phycoerythrin (R-PE) and the conjugate isolated by size exclusion chromatography. Product is at 90 ug/ml. A567/A280 = 3.7.
Buffer
10 mM PBS pH 7.4, 1% BSA, 0.15% Sodium Azide (as a preservative).
Group Header
Streptavidin
Preparation and Storage
Store at 2 - 5 degree C. Do not freeze! Protect from light.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Related Product Information for anti-Streptavidin antibody
Information: Streptavidin R-PE can be used in conjunction with biotinylated antibodies as an avidin/biotin labeling system for flow cytometry.
NCBI and Uniprot Product Information
Similar Products
Product Notes
The Streptavidin (Catalog #AAA666773) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Streptavidin can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). 1:10 (or use 2 ul of concentrated stock per 5 x 105-cell test). Researchers should empirically determine the suitability of the Streptavidin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Streptavidin, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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