Testing Data
Hamster anti-Mouse CD3 Monoclonal Antibody | anti-CD3 antibody
anti-mouse CD3-APC
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot, ELISA
Synonyms
CD3; Monoclonal Antibody; anti-mouse CD3-APC; Monoclonal anti-mouse CD3/APC; anti-CD3 antibody
Host
Hamster
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG
Clone Number
145-2C11
Form/Format
50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA.
Concentration
Volume: 0.2ml (varies by lot)
Sequence Length
167
Applicable Applications for anti-CD3 antibody
Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB), ELISA (EIA)
Application Notes
1:50 (or use 1.6ul of concentrated stock per 5 x 105-cell test)
Performance
Reagent was tested for binding to ACK lysed murine splenocytes in FACS. Five x 105 splenocytes per tube were washed and pre incubated with 20 ul of 300 ug/ml Mouse IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 ul of anti-Mouse CD3/APC at a 1:50 dilution (5 ug/ml). They were then washed three times, fixed and analyzed by FACS. A 44.4% sub population of the cells stained positive with a mean shift of 1.35 log10 fluorescent units when compared to background. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-mouse CD3 antibody.
Conjugation
APC
Production
Protein G purified antibody from tissue culture supernatant was conjugated to Allophycocycanin through a sulfo-ester linkage. Unconjugated antibody was removed using size exclusion chromatography. Antibody conjugate is at 250 ug/ml with an A650/A280 ratio of 2.26.
Buffer
50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative)
Immunogen
Mouse BM10-37 T cell membrane prep
Preparation and Storage
Store at 2 - 5 degree C. Do not freeze! Protect from light.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Testing Data
Testing Data
Related Product Information for anti-CD3 antibody
Information: Antibody 145-2C11binds to an extracellular epitope of the 25 kd CD3e molecule present on murine T cells (1). CD3 molecules associate with T cell Receptors and provide intracellular signaling mechanism. Cross linking Cell surface CD3 with soluble or immobilized 145-2C11 can activate this mechanism(2).
References
1) O Leo, J A Bluestone, et al. (1987) PNAS USA 84(5): 1374-1378. 2) L E Samelson, J A Bluestone, et al. (1987) J Immunol 139(8): 2708-2714.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
18,897 Da
NCBI Official Full Name
cd3
UniProt Protein Name
CD3gamma/delta
Protein Family
UniProt Gene Name
cd3g
UniProt Entry Name
P79951_XENLA
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Product Notes
The CD3 cd3g (Catalog #AAA666074) is an Antibody produced from Hamster and is intended for research purposes only. The product is available for immediate purchase. The anti-mouse CD3-APC reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD3 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB), ELISA (EIA). 1:50 (or use 1.6ul of concentrated stock per 5 x 105-cell test). Researchers should empirically determine the suitability of the CD3 cd3g for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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