Rabbit anti-Human PCBP1 Polyclonal Antibody | anti-PCBP1 antibody

PCBP1, ID (PCBP1, Poly(rC)-binding protein 1, Alpha-CP1, Heterogeneous nuclear ribonucleoprotein E1, Nucleic acid-binding protein SUB2.3) (FITC)

Cat. Num. AAA6331333

• Gene Names: PCBP1; HNRPX; HNRPE1; hnRNP-X; HEL-S-85; hnRNP-E1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PCBP1; Polyclonal Antibody; ID (PCBP1; Poly(rC)-binding protein 1; Alpha-CP1; Heterogeneous nuclear ribonucleoprotein E1; Nucleic acid-binding protein SUB2.3) (FITC); Nucleic acid-binding protein SUB2.3; anti-PCBP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-PCBP1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PCBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic between 187-217 amino acids selected from the Central region of human PCBP1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PCBP1 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PCBP1 antibody detected the PCBP1 protein (arrow).)

Related Product Information for anti-PCBP1 antibody

This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability.

Product Categories/Family for anti-PCBP1 antibody

Antibodies; Nuclear Proteins

References

Cloke, B., et al. Endocrinology 151(8):3954-3964(2010). Wang, H., et al. Cancer Cell 18(1):52-62(2010). Zhang, T., et al. Mol. Cancer 9, 72 (2010): Waggoner, S.A., et al. J. Biol. Chem. 284(14):9039-9049(2009). Huo, L.R., et al. Biochim. Biophys. Acta 1784(11):1524-1533(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 222352151
• NCBI GeneID: 5093
• NCBI Accession #: NP_006187.2
• NCBI GenBank Nucleotide #: NM_006196.3
• UniProt Accession #: Q15365; Q13157; Q14975
• Molecular Weight: 37,498 Da
• NCBI Official Full Name: poly(rC)-binding protein 1
• NCBI Official Synonym Full Names: poly(rC) binding protein 1
• NCBI Official Symbol: PCBP1
• NCBI Official Synonym Symbols: HNRPX; HNRPE1; hnRNP-X; HEL-S-85; hnRNP-E1
• NCBI Protein Information: poly(rC)-binding protein 1; poly(rC)-binding protein 1; alpha-CP1; epididymis secretory protein Li 85; heterogeneous nuclear ribonucleoprotein E1; heterogenous nuclear ribonucleoprotein E1; heterogenous nuclear ribonucleoprotein X; nucleic acid-binding pr
• UniProt Protein Name: Poly(rC)-binding protein 1
• Protein Family: Poly(rC)-binding protein
• UniProt Gene Name: PCBP1
• UniProt Synonym Gene Names: hnRNP E1
• UniProt Entry Name: PCBP1_HUMAN

NCBI Description

This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. [provided by RefSeq, Jul 2008]

Uniprot Description

hnRNP E1: Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. Abundantly expressed in skeletal muscle, thymus and peripheral blood leukocytes while a lower expression is observed in prostate, spleen, testis, ovary, small intestine, heart, liver, adrenal and thyroid glands.

Protein type: RNA splicing; Translation; RNA-binding

Chromosomal Location of Human Ortholog: 2p13-p12

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; membrane; cytoplasm; ribonucleoprotein complex

Molecular Function: protein binding; RNA binding; single-stranded DNA binding

Biological Process: nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression