Chicken anti-Rat Internexin, alpha Polyclonal Antibody | anti-INA antibody

Internexin, alpha (alpha-Internexin, alpha-Inx, Ina, Inexa, Neurofilament-66, NF66)

Cat. Num. AAA647249

• Gene Names: INA; NEF5; NF-66; TXBP-1
• Reactivity: Rat
• Applications: Western Blot, Immunofluorescence
• Purity: Purified; Purified
• Synonyms: Internexin; alpha; Polyclonal Antibody; alpha (alpha-Internexin; alpha-Inx; Ina; Inexa; Neurofilament-66; NF66); Anti -Internexin; anti-INA antibody

• Host: Chicken
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgY
• Specificity: Recognizes rat a-Internexin.
• Purity/Purification: Purified; Purified
• Form/Format: Supplied as a liquid.

• Applicable Applications for anti-INA antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence and Western Blot.; Dilution: Immunofluorescence: 1:1000; Western Blot: 1:10,000; On Western Blots use a brain or spinal cord homogenate and look for a major band at SDS-PAGE apparent molecular weight of 66 to 68kD. The human protein runs at ~68kD, while rat, mouse and other species run at ~66kD. Minor bands at ~150kD are probably covalent dimers, and bands at ~50kD represent a-internexin breakdown products.
• Immunogen: Full length cDNA encoding rat alpha-internexin
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot of extract of rat brain stem crude extract stained with MBS647249, showing a single strong clean band at ~66kD.)

Testing Data

(Mixed neuron glia cultures stain with MBS647249 (red) and counterstained with 031354 rabbit polyclonal antibody to Coronin 1a, (green) which is an excellent marker of microglia and lymphocytes. The chicken internexin antibody is an excellent marker of neuronal processes in these cultures.)

Related Product Information for anti-INA antibody

Neurofilaments can be defined as the intermediate or 10nm diameter filaments found in neuronal cells. They are composed a mixture of subunits which often includes triplet proteins, NF-L, NF-M and NF-H. Neurofilaments may also include peripherin, alpha-internexin, nestin and in some cases vimentin. a-internexin is a ~66kD Class IV intermediate filament originally discovered as it copurifies with other neurofilament subunits (1). It is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. It is expressed in large amounts early in neuronal development, but is downregulated in many neurons as development procedes. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to alpha-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of alpha-internexin means its presence is an early and convenient feature of neuronal progenitors cells and other cell committed to the neuronal lineage. In addition recent studies show a marked up-regulation of alpha-internexin during neuronal regeneration (2), and that alpha-internexin positive inclusions are the hallmark of a type of frontotemporal dementia known as neurofilament inclusion body disease (3). The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest. The HGNC name for this protein is INA.

Product Categories/Family for anti-INA antibody

Antibodies; Abs to Neurofilament

NCBI and Uniprot Product Information

• NCBI GI #: 119570039
• NCBI GeneID: 9118
• UniProt Accession #: Q16352; Q9BRC5; B1AQK0
• Molecular Weight: 55,391 Da
• NCBI Official Full Name: internexin neuronal intermediate filament protein, alpha, isoform CRA_b
• NCBI Official Synonym Full Names: internexin neuronal intermediate filament protein, alpha
• NCBI Official Symbol: INA
• NCBI Official Synonym Symbols: NEF5; NF-66; TXBP-1
• NCBI Protein Information: alpha-internexin; alpha-Inx; neurofilament 5 (66kD); 66 kDa neurofilament protein; neurofilament-66, tax-binding protein
• UniProt Protein Name: Alpha-internexin
• Protein Family: Immune inhibitor
• UniProt Gene Name: INA
• UniProt Synonym Gene Names: NEF5; Alpha-Inx; NF-66
• UniProt Entry Name: AINX_HUMAN

NCBI Description

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene is a member of the intermediate filament family and is involved in the morphogenesis of neurons. [provided by RefSeq, Jun 2009]

Uniprot Description

Function: Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges.

Tissue specificity: Found predominantly in adult CNS.

Developmental stage: Expressed in brain as early as the 16th week of gestation, and increased rapidly and reached a steady state level by the 18th week of gestation.

Post-translational modification: O-glycosylated

By similarity.

Sequence similarities: Belongs to the intermediate filament family.