Goat anti-Mouse Apolipoprotein AI Polyclonal Antibody | anti-Apoa1 antibody

Apolipoprotein AI (Apo AI) (AP)

Cat. Num. AAA6463601

• Gene Names: Apoa1; Sep2; Alp-1; Ltw-1; Sep-1; Sep-2; Apoa-1; Brp-14; Lvtw-1; apo-AI; apoA-I
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: Apolipoprotein AI; Polyclonal Antibody; Apolipoprotein AI (Apo AI) (AP); anti-Apoa1 antibody

• Host: Goat
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse Apolipoprotein AI (Apo AI). Minimum cross-reactivity to Apo AII, Apo B, Apo CI, Apo CII, Apo CIII, Apo E and Apo J. Non-specific crossreaction with other mouse serum proteins is negligible.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-Apoa1 antibody: Western Blot (WB)
• Application Notes: WB: 1:250-1:500; Applications are based on unconjugated antibody.; ELISA: 1:2500-1:5000. Assayed against 1ug of Apolipoprotein in a standard ELISA using (IgG, H&L (HRP)) RbxGt and ABTS (A0030) for 30 minutes at RT.
• Immunogen: Apo AI isolated from mouse plasma.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C before opening. DO NOT FREEZE! Stable at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing alkaline phosphatase conjugates will result in a substantial loss of activity.

Product Categories/Family for anti-Apoa1 antibody

Antibodies; Lipoproteins

NCBI and Uniprot Product Information

• NCBI GI #: 160333304
• NCBI GeneID: 11806
• NCBI Accession #: NP_033822.2
• NCBI GenBank Nucleotide #: NM_009692.4
• UniProt Accession #: Q00623; O08855; O09042; Q8BPD5
• Molecular Weight: 30,616 Da
• NCBI Official Full Name: apolipoprotein A-I preproprotein
• NCBI Official Synonym Full Names: apolipoprotein A-I
• NCBI Official Symbol: Apoa1
• NCBI Official Synonym Symbols: Sep2; Alp-1; Ltw-1; Sep-1; Sep-2; Apoa-1; Brp-14; Lvtw-1; apo-AI; apoA-I
• NCBI Protein Information: apolipoprotein A-I
• UniProt Protein Name: Apolipoprotein A-I
• Protein Family: Apolipoprotein
• UniProt Gene Name: Apoa1
• UniProt Synonym Gene Names: Apo-AI; ApoA-I; ProapoA-I
• UniProt Entry Name: APOA1_MOUSE

Uniprot Description

APOA1: Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility. Interacts with APOA1BP and CLU. Component of a sperm activating protein complex (SPAP), consisting of APOA1, an immunoglobulin heavy chain, an immunoglobulin light chain and albumin. Interacts with NDRG1. Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. The oxidized form at Met-110 and Met-136 is increased in individuals with increased risk for coronary artery disease, such as in carrier of the eNOSa/b genotype and exposure to cigarette smoking. It is also present in increased levels in aortic lesions relative to native ApoA-I and increased levels are seen with increasing severity of disease. Belongs to the apolipoprotein A1/A4/E family.

Protein type: Cell development/differentiation; Endoplasmic reticulum; Lipid-binding; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide; Vesicle

Cellular Component: cell surface; chylomicron; cytoplasmic vesicle; endocytic vesicle; extracellular region; extracellular space; nucleus

Molecular Function: apolipoprotein A-I receptor binding; apolipoprotein receptor binding; beta-amyloid binding; chemorepellent activity; cholesterol binding; cholesterol transporter activity; enzyme binding; high-density lipoprotein binding; identical protein binding; lipase inhibitor activity; lipid binding; lipid transporter activity; phosphatidylcholine binding; phospholipid binding; phospholipid transporter activity; protein binding

Biological Process: adrenal gland development; axon regeneration; blood vessel endothelial cell migration; cholesterol biosynthetic process; cholesterol efflux; cholesterol homeostasis; cholesterol metabolic process; cholesterol transport; endothelial cell proliferation; G-protein coupled receptor protein signaling pathway; glucocorticoid metabolic process; integrin-mediated signaling pathway; lipoprotein biosynthetic process; negative chemotaxis; negative regulation of cytokine secretion during immune response; negative regulation of heterotypic cell-cell adhesion; negative regulation of hydrolase activity; negative regulation of inflammatory response; negative regulation of interleukin-1 beta secretion; negative regulation of lipase activity; organ regeneration; peptidyl-methionine modification; phosphatidylcholine biosynthetic process; phosphatidylcholine metabolic process; phospholipid efflux; phospholipid homeostasis; phospholipid metabolic process; phospholipid transport; positive regulation of fatty acid biosynthetic process; positive regulation of hydrolase activity; positive regulation of lipoprotein lipase activity; positive regulation of Rho protein signal transduction; positive regulation of stress fiber formation; positive regulation of transferase activity; protein amino acid oxidation; protein localization; protein stabilization; regulation of Cdc42 protein signal transduction; regulation of cholesterol absorption; regulation of protein amino acid phosphorylation; reverse cholesterol transport; sequestering of lipid; triacylglycerol catabolic process; vitamin transport