Mouse apolipoprotein A-I ELISA Kit | APOA1 elisa kit

Mouse apolipoprotein A1, Apo-A1 ELISA Kit

Cat. Num. AAA702111

• Gene Names: Apoa1; Sep2; Alp-1; Ltw-1; Sep-1; Sep-2; Apoa-1; Brp-14; Lvtw-1; apo-AI; apoA-I
• Reactivity: Mouse
• Synonyms: apolipoprotein A-I; Mouse apolipoprotein A1; Apo-A1 ELISA Kit; Mouse apolipoprotein A1 (Apo-A1) ELISA Kit; MGC117399; OTTHUMP00000069346; OTTHUMP00000069347; OTTHUMP00000069348; APOA1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse Apo-A1. No significant cross-reactivity or interference between mouse Apo-A1 and analogues was observed.
• Sequence Length: 264

• Samples: Serum, plasma, cell culture supernates, tissue homogenates, urine.
• Assay Type: Sandwich (Quantitative)
• Detection Range: 31.25 ng/ml-2000 ng/ml.
• Sensitivity: 7.81 ng/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for APOA1 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Apo-A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Apo-A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Apo-A1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Apo-A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 160333304
• NCBI GeneID: 11806
• NCBI Accession #: NP_033822.2
• NCBI GenBank Nucleotide #: NM_009692.4
• UniProt Accession #: Q00623; O08855; O09042; Q8BPD5
• Molecular Weight: 30,616 Da
• NCBI Official Full Name: apolipoprotein A-I preproprotein
• NCBI Official Synonym Full Names: apolipoprotein A-I
• NCBI Official Symbol: Apoa1
• NCBI Official Synonym Symbols: Sep2; Alp-1; Ltw-1; Sep-1; Sep-2; Apoa-1; Brp-14; Lvtw-1; apo-AI; apoA-I
• NCBI Protein Information: apolipoprotein A-I; apolipoprotein A1
• UniProt Protein Name: Apolipoprotein A-I
• Protein Family: Apolipoprotein
• UniProt Gene Name: Apoa1
• UniProt Synonym Gene Names: Apo-AI; ApoA-I; ProapoA-I
• UniProt Entry Name: APOA1_MOUSE

NCBI Description

This gene encodes a preproprotein that is proteolytically cleaved to yield a signal peptide and a proproptein that is subsequently processed to generate the active mature peptide. The encoded protein is the major protein component of plasma high density lipoprotein (HDL). This protein facilitates the removal of cholesterol and other fats from tissues by transporting them to the liver for excretion. This protein is a cofactor for lecithin cholesterolacyltransferase, an enzyme that catalyzes the conversion of free cholesterol to cholesteryl esters. Mutations in this gene in humans causes familial HDL deficiency, Tangier disease and familial visceral amyloidosis. Similar clinical features are exhibited by mice with mutations in this gene. This gene is clustered with three other apolipoprotein genes on chromosome 9. [provided by RefSeq, Dec 2013]

Uniprot Description

Function: Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.