Syndecan-1 Recombinant Protein | SDC1 recombinant protein

Human CellExp Syndecan-1 Protein

Cat. Num. AAA845573

• Gene Names: SDC1; SDC; CD138; SYND1; syndecan
• Applications: SDS-Page
• Purity: >92% by SDS-PAGE
• Synonyms: Syndecan-1; Human CellExp Syndecan-1 Protein; SDC1; CD138; SYND1; SDC; SDC1 recombinant protein

• Host: Human
• Purity/Purification: >92% by SDS-PAGE
• Form/Format: Lyophilized; Lyophilized from 0.22 um filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
• Sequence Positions: Gln 23 - Gly 254

• Applicable Applications for SDC1 recombinant protein: SDS-PAGE
• Source: HEK 293 cells
• Endotoxin Level: < 1.0 EU per ug of the protein by the LAL method.
• Reconstitution: Centrifuge the vial prior to opening. Reconstitute in sterile deionized water to a stock solution of 200 ug/mL. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% (W/V) HSA or BSA) is recommended for further dilution and long term storage.
• Preparation and Storage: Store at 4°C for up to 1 year in lyophilized. After reconstitution, aliquot and store at -70°C for up to 3 months. Avoid repeated freezing and thawing cycles.

Testing Data

(The purity of Human Syndecan-1 protein was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.)

Related Product Information for SDC1 recombinant protein

Syndecan-1 (SYND1 or SDC1) is also known as CD antigen CD138; is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding; cell signaling; and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1/SDC1 protein functions as an integral membrane protein and participates in cell proliferation; cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a useful marker for plasma cells; but only if the cells tested are already known to be derived from blood.

NCBI and Uniprot Product Information

• NCBI GI #: 55749480
• NCBI GeneID: 6382
• NCBI Accession #: NP_001006947.1
• NCBI GenBank Nucleotide #: NM_001006946.1
• UniProt Accession #: P18827; Q53QV0; Q546D3; Q96HB7; D6W523; AAB21434; AAB23935
• Molecular Weight: This protein is fused with polyhistidine tag at the C-terminus, has a calculated MW of 24.7 kDa. The predicted N-terminus is Gln 23. DTT-reduced Protein migrates as 48-60 kDa due to glycosylation.
• NCBI Official Full Name: syndecan-1
• NCBI Official Synonym Full Names: syndecan 1
• NCBI Official Symbol: SDC1
• NCBI Official Synonym Symbols: SDC; CD138; SYND1; syndecan
• NCBI Protein Information: syndecan-1
• UniProt Protein Name: Syndecan-1
• Protein Family: Syndecan
• UniProt Gene Name: SDC1
• UniProt Synonym Gene Names: SDC; SYND1

NCBI Description

The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

syndecan-1: Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. Belongs to the syndecan proteoglycan family.

Protein type: Extracellular matrix; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2p24.1

Cellular Component: cell surface; Golgi lumen; integral to plasma membrane; lysosomal lumen; plasma membrane

Molecular Function: protein binding; protein C-terminus binding

Biological Process: cell migration; glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; glycosaminoglycan metabolic process; leukocyte migration; myoblast development; retinoid metabolic process; striated muscle cell development