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Testing Data ()

Mbo I Restriction Enzyme | mbo restriction enzyme

Mbo I

Gene Names
mbo; 1465/07; 1465/7; anon-WO0118547.263; CG6819; DmelCG6819; Dnup 88; dNup88; Dnup88; DNup88; l(3)05043; l(3)S146507; Mbo; nup88; Nup88
Purity
Molecular Biology Grade
Synonyms
Mbo I; mbo restriction enzyme
Ordering
For Research Use Only!
Host
Moraxella bovis
Purity/Purification
Molecular Biology Grade
Dilution Buffer
10mM Tris-HCl (pH 7.4 at 25 degree C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. For longer periods the Storage Buffer should be used.
Compatible Ends
BamHI, BclI, BglII, PsuI
Methylation Effects
MboI cuts neither Gm6ATC, GAThm5C nor GAhm5UC, but cuts GATm4C and GATm5C. Blocked by Dam methylation.
pACYC177
22
pACYC184
15
Labeled Oligonucleotide (LO) Assay
No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours.
Lambda
116
Ligation/Recutting Assay
After 50-fold overdigestion (3u/ug DNA x 17 hours) with MboI, more than 90% of the DNA dam-, fragments can be ligated at a 5'-termini concentration of 1.2uM. More than 90% of these can be recut.
M13mp18/19
7
Overdigestion Assay
No detectable change in the specific fragmentation pattern is observed after 320-fold overdigestion (20u/ug lambda DNA dam-x 16 hours) with MboI.
pBR322
22
pTZ19R/U
15
pUC18/19
15
pUC57
15
Stability During Prolonged Incubation
A minimum of 0.1units of enzyme are required for complete digestion of 1ug of lambda DNA in 16 hours at 37 degree C.
Storage Buffer
10mM Tris-HCl (pH 7.5 at 25 degree C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
Thermal Inactivation
Enzyme is inactivated by incubation at 65 degree C for 20min.
Unit Definition
One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA dam-in 1 hour at 37 degree C in 50ul of assay buffer.
Preparation and Storage
-20 degree C

Testing Data

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Testing Data ()
Related Product Information for mbo restriction enzyme
5'-^G A T C-3'
Product Categories/Family for mbo restriction enzyme

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
78,681 Da
NCBI Official Full Name
members only
NCBI Official Synonym Full Names
members only
NCBI Official Symbol
mbo
NCBI Official Synonym Symbols
1465/07; 1465/7; anon-WO0118547.263; CG6819; DmelCG6819; Dnup 88; dNup88; Dnup88; DNup88; l(3)05043; l(3)S146507; Mbo; nup88; Nup88
NCBI Protein Information
CG6819 gene product from transcript CG6819-RA; CG6819-PA; mbo-PA; members only
UniProt Protein Name
Nuclear pore complex protein Nup88
UniProt Gene Name
mbo
UniProt Synonym Gene Names
Nup88
UniProt Entry Name
NUP88_DROME

Uniprot Description

Function: Essential component of nuclear pore complex. Selectively required for the nuclear import of the Rel family transcription factors dorsal (dl) and Dorsal-related immunity factor (Dif) and the activation of an immune response. Required for the anchoring of Nup214 and emb on the nuclear envelope and thereby attenuates nuclear export signal (NES)-mediated nuclear export. Ref.1 Ref.5

Subunit structure: Interacts with Nup214 and emb to form components of the nuclear pore complex. Interacts with tamo for nuclear import of target proteins including dl, msl-1, ran and ran-like. Ref.5 Ref.6

Subcellular location: Nucleus › nuclear pore complex. Nucleus membrane; Peripheral membrane protein; Cytoplasmic side Ref.1.

Tissue specificity: In the embryo, expression is prominent in a subset of the cells of the developing CNS, dynamic stripes of epidermal cells, lymph glands and intestinal tract including the proventriculus and foregut. In the larva, expression was seen in the fusion cells of the trachea, fat body, imaginal tissues, and with abundance in the proliferating parts of the nerve cord, optic lobes of the brain and imaginal disks. Ref.1

Developmental stage: Expressed both maternally and zygotically. Ref.1

Research Articles on mbo

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Product Notes

The mbo mbo (Catalog #AAA638410) is a Restriction Enzyme produced from Moraxella bovis and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Mbo I, Restriction Enzyme" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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