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Western Blot (WB) (Western Blot analysis of MBS629244 in Hela,HepG2 cell line lysates (35ug/lane).This demonstrates that MBS629244 detected the LRPAP1 protein (arrow).)

Rabbit anti-Human Lipoprotein Receptor-related Protein Associated Protein 1, Low Density Polyclonal Antibody | anti-LRPAP1 antibody

Lipoprotein Receptor-related Protein Associated Protein 1, Low Density, CT (Low Density Lipoprotein Receptor-related Protein Associated Protein 1, LRPAP1, Alpha-2-macroglobulin Receptor-associated Protein, Alpha-2-MRAP, A2MRAP, A2RAP, HBP44, MGC138272, MR

Gene Names
LRPAP1; RAP; MRAP; A2RAP; HBP44; A2MRAP
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Purified by Protein A and Peptide Affinity Chromatography
Synonyms
Lipoprotein Receptor-related Protein Associated Protein 1; Low Density; Polyclonal Antibody; CT (Low Density Lipoprotein Receptor-related Protein Associated Protein 1; LRPAP1; Alpha-2-macroglobulin Receptor-associated Protein; Alpha-2-MRAP; A2MRAP; A2RAP; HBP44; MGC138272; MR; Lipoprotein Receptor Associated Protein; anti-LRPAP1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human LRPAP1.
Purity/Purification
Purified by Protein A and Peptide Affinity Chromatography
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.
Applicable Applications for anti-LRPAP1 antibody
FLISA, Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 281-309 in the C-terminal region of human LRPAP1, UniProt Accession #P30533
Conjugate
MaxLight650
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of MBS629244 in Hela,HepG2 cell line lysates (35ug/lane).This demonstrates that MBS629244 detected the LRPAP1 protein (arrow).)

Western Blot (WB) (Western Blot analysis of MBS629244 in Hela,HepG2 cell line lysates (35ug/lane).This demonstrates that MBS629244 detected the LRPAP1 protein (arrow).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue withMBS629244, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS629244 for immunohistochemistry.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human brain tissue withMBS629244, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS629244 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of HepG2 cells using MBS629244 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (Flow cytometric analysis of HepG2 cells using MBS629244 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-LRPAP1 antibody
LRPAP1 interacts with LRP1/alpha-2-macroglobulin receptor and glycoprotein 330.
Product Categories/Family for anti-LRPAP1 antibody
References
1. Lisi, S., et.al., J. Endocrinol. Invest. 30(10), 839-843 (2007). 2. Vertegaal, A.C., et.al., Mol. Cell Proteomics 5(12), 2298-2310 (2006).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
160,000
NCBI Official Full Name
alpha-2-macroglobulin receptor-associated protein
NCBI Official Synonym Full Names
low density lipoprotein receptor-related protein associated protein 1
NCBI Official Symbol
LRPAP1
NCBI Official Synonym Symbols
RAP; MRAP; A2RAP; HBP44; A2MRAP
NCBI Protein Information
alpha-2-macroglobulin receptor-associated protein; alpha-2-MRAP; lipoprotein receptor associated protein; alpha-2-macroglobulin receptor-associated protein 1; low density lipoprotein receptor-related protein-associated protein 1; low density lipoprotein-r
UniProt Protein Name
Alpha-2-macroglobulin receptor-associated protein
UniProt Gene Name
LRPAP1
UniProt Synonym Gene Names
A2MRAP; Alpha-2-MRAP; RAP
UniProt Entry Name
AMRP_HUMAN

NCBI Description

This gene encodes a protein that interacts with the low density lipoprotein (LDL) receptor-related protein and facilitates its proper folding and localization by preventing the binding of ligands. Mutations in this gene have been identified in individuals with myopia 23. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]

Uniprot Description

LRPAP1: Interacts with LRP1/alpha-2-macroglobulin receptor and glycoprotein 330. In complex with the alpha-2-MR or gp330, it may have some role in the pathogenesis of membrane glomerular nephritis. Belongs to the alpha-2-MRAP family.

Protein type: Receptor, misc.

Chromosomal Location of Human Ortholog: 4p16.3

Cellular Component: cell surface; endoplasmic reticulum; extracellular region; plasma membrane; integral to membrane; vesicle

Molecular Function: heparin binding; receptor antagonist activity; protein binding; low-density lipoprotein receptor binding; unfolded protein binding; asialoglycoprotein receptor activity

Biological Process: vesicle-mediated transport; receptor-mediated endocytosis; protein folding; negative regulation of protein binding

Disease: Myopia 23, Autosomal Recessive

Research Articles on LRPAP1

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Product Notes

The LRPAP1 lrpap1 (Catalog #AAA6485298) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Lipoprotein Receptor-related Protein Associated Protein 1, Low Density, CT (Low Density Lipoprotein Receptor-related Protein Associated Protein 1, LRPAP1, Alpha-2-macroglobulin Receptor-associated Protein, Alpha-2-MRAP, A2MRAP, A2RAP, HBP44, MGC138272, MR reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Lipoprotein Receptor-related Protein Associated Protein 1, Low Density can be used in a range of immunoassay formats including, but not limited to, FLISA, Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the LRPAP1 lrpap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Lipoprotein Receptor-related Protein Associated Protein 1, Low Density, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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