CaiI Restriction Enzyme | CA2 restriction enzyme

CaiI (AlwNI)

Cat. Num. AAA638343

• Gene Names: CA2; CAC; CAII; Car2; CA-II
• Purity: Molecular Biology Grade
• Synonyms: CaiI; CaiI (AlwNI); CA2 restriction enzyme

• Host: Comamonas acidovorans Lti 19-021
• Purity/Purification: Molecular Biology Grade

• Dilution Buffer: 10mM Tris-HCl (pH 7.4 at 25 degree C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
• Digestion of Agarose-embedded DNA: A minimum of 5units of enzyme is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours.
• Methylation Effects: CaiI does not cut CAGNNCm5CTG. Blocked by overlapping Dcm methylation.
• pACYC177: 1
• pACYC184: 3
• Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10units of restriction endonuclease for 4 hours.
• Lambda: 41
• Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with CaiI, more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 0.3uM. More than 95% of these can be recut.
• M13mp18/19: 1
• Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 320-fold overdigestion (20u/ug lambda DNA x 16 hours) with CaiI.
• pBR322: 1
• pTZ19R/U: 1
• pUC18/19: 1
• pUC57: 1
• Stability During Prolonged Incubation: A minimum of 0.2units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37 degree C.
• Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25 degree C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
• Thermal Inactivation: Enzyme is inactivated by incubation at 65 degree C for 20min.
• Unit Definition: One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37 degree C in 50ul of assay buffer.
• Preparation and Storage: -20 degree C

Testing Data

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Related Product Information for CA2 restriction enzyme

5'-C A G N N N^C T G-3'

Product Categories/Family for CA2 restriction enzyme

Molecular Biology; MB-Enzymes; Restriction

NCBI and Uniprot Product Information

• NCBI GI #: 4557395
• NCBI GeneID: 760
• NCBI Accession #: NP_000058.1
• NCBI GenBank Nucleotide #: NM_000067.2
• UniProt Accession #: P00918; Q6FI12; Q96ET9; B2R7G8
• Molecular Weight: 29,246 Da
• NCBI Official Full Name: carbonic anhydrase 2
• NCBI Official Synonym Full Names: carbonic anhydrase II
• NCBI Official Symbol: CA2
• NCBI Official Synonym Symbols: CAC; CAII; Car2; CA-II
• NCBI Protein Information: carbonic anhydrase 2; carbonic anhydrase B; carbonic anhydrase C; carbonic dehydratase; carbonate dehydratase II
• UniProt Protein Name: Carbonic anhydrase 2
• Protein Family: Beta-carotene hydroxylase
• UniProt Gene Name: CA2
• UniProt Synonym Gene Names: CAC; CA-II
• UniProt Entry Name: CAH2_HUMAN

NCBI Description

CA2 is one of several (at least 7) isozymes of carbonic anhydrase. Carbonic anhydrase catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. [provided by RefSeq, Jul 2008]

Uniprot Description

CA2: Essential for bone resorption and osteoclast differentiation. Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Interacts with SLC4A4. Interaction with SLC4A7 regulates SLC4A7 transporter activity. Activated by X-ray, histamine, L-adrenaline, L- and D-phenylalanine, L- and D-histidine, L-His-OMe and beta-Ala- His (carnosine). Competitively inhibited by saccharin, thioxolone, coumarins, 667-coumate, celecoxib (Celebrex), valdecoxib (Bextra), SC-125, SC-560, diclofenac, acetate, azide, bromide, sulfonamide derivatives such as acetazolamide (AZA), methazolamide (MZA), ethoxzolamide (EZA), dichlorophenamide (DCP), brinzolamide, dansylamide, thiabendazole-5-sulfonamide, trifluoromethane sulfonamide and N-hydroxysulfamide, fructose-based sugar sulfamate RWJ-37497, and Foscarnet (phosphonoformate trisodium salt). Repressed strongly by hydrogen sulfide(HS) and weakly by nitrate (NO(3)). Esterase activity weakly reduced by cyanamide. N- hydroxyurea interfers with zinc binding and inhibit activity. Belongs to the alpha-carbonic anhydrase family.

Protein type: Energy Metabolism - nitrogen; EC 4.2.1.1; Lyase

Chromosomal Location of Human Ortholog: 8q22

Cellular Component: extracellular space; microvillus; basolateral plasma membrane; apical part of cell; axon; cytoplasm; plasma membrane; cytosol; myelin sheath

Molecular Function: protein binding; carbonate dehydratase activity; zinc ion binding

Biological Process: secretion; positive regulation of osteoclast differentiation; positive regulation of cellular pH reduction; positive regulation of synaptic transmission, GABAergic; one-carbon compound metabolic process; odontogenesis of dentine-containing teeth; response to zinc ion; response to estrogen stimulus; bicarbonate transport; regulation of intracellular pH; positive regulation of bone resorption; morphogenesis of an epithelium; kidney development; response to pH

Disease: Osteopetrosis, Autosomal Recessive 3