Rabbit anti-Human Fetoprotein, Alpha Polyclonal Antibody | anti-FETA antibody

Fetoprotein, Alpha (AFP, Alpha1-fetoprotein, Alpha-fetoglobulin, Alpha-fetoprotein precursor, FETA, HPAFP) (AP)

Cat. Num. AAA6368989

• Gene Names: AFP; AFPD; FETA; HPAFP
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Fetoprotein; Alpha; Polyclonal Antibody; Alpha (AFP; Alpha1-fetoprotein; Alpha-fetoglobulin; Alpha-fetoprotein precursor; FETA; HPAFP) (AP); anti-FETA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human AFP.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-FETA antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human AFP, aa1-609 (AAH27881.1).
• Immunogen Sequence: MKWVESIFLIFLLNFTESRTLHRNEYGIASILDSYQCTAEISLADLATIFFAQFVQEATYKEVSKMVKDALTAIEKPTGDEQSSGCLENQLPAFLEELCHEKEILEKYGHSDCCSQSEEGRHNCFLAHKKPTPASIPLFQVPEPVTSCEAYEEDRETFMNKFIYEIARRHPFLYAPTILLWAARYDKIIPSCCKAENAVECFQTKAATVTKELRESSLLNQHACAVMKNFGTRTFQAITVTKLSQKFTKVNFTEIQKLVLDVAHVHEHCCRGDVLDCLQDGEKIMSYICSQQDTLSNKITECCKLTTLERGQCIIHAENDEKPEGLSPNLNRFLGDRDFNQFSSGEKNIFLASFVHEYSRRHPQLAVSVILRVAKGYQELLEKCFQTENPLECQDKGEEELQKYIQESQALAKRSCGLFQKLGEYYLQNAFLVAYTKKAPQLTSSELMAITRKMAATAATCCQLSEDKLLACGEGAADIIIGHLCIRHEMTPVNPGVGQCCTSSYANRRPCFSSLVVDETYVPPAFSDDKFIFHKDLCQAQGVALQTMKQEFLINLVKQKPQITEEQLEAVIADFSGLLEKCCQGQEQEVCFAEEGQKLISKTRAALGV
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of AFP expression in transfected 293T cell line by AFP polyclonal antibody. Lane 1: AFP transfected lysate (68.7kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-FETA antibody

Alpha-fetoprotein (AFP) is a serum glycoprotein protein produced in the liver or yolk sac of fetal staged mammals. AFP synthesis is minimal after birth and trace amount is expressed in the adult liver. AFP gene expression is regulated by the interactions between steroid hormone receptors and transcriptional factors in separate signal transduction pathways. AFP functions as a binding and transporting ligand and cell growth regulator. An elevated expression level of AFP has been implicated in colorectal, ovarian, pancreatic, testicular, and certain liver cancers. High level of AFP is also seen some diseases, such as hepatitis and colitis. AFP is used as a screening marker for fetal abnormalities in pregnant women, such as Down syndrome. Recently, AFP has been introduced as an anti-cancer drug-ligand carrier, transporting drugs to target tumor cells, increasing anti-tumor efficiency.

Product Categories/Family for anti-FETA antibody

Antibodies; Serum Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 20379787
• NCBI GeneID: 174
• NCBI Accession #: AAH27881.1
• NCBI GenBank Nucleotide #: NM_001134
• UniProt Accession #: P02771; B2RBU3
• Molecular Weight: 68,678 Da
• NCBI Official Full Name: Alpha-fetoprotein
• NCBI Official Synonym Full Names: alpha-fetoprotein
• NCBI Official Symbol: AFP
• NCBI Official Synonym Symbols: AFPD; FETA; HPAFP
• NCBI Protein Information: alpha-fetoprotein
• UniProt Protein Name: Alpha-fetoprotein
• Protein Family: Probable iron export ATP-binding protein
• UniProt Gene Name: AFP
• UniProt Synonym Gene Names: HPAFP
• UniProt Entry Name: FETA_HUMAN

NCBI Description

This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.

Subunit structure: Dimeric and trimeric forms have been found in addition to the monomeric form.

Subcellular location: Secreted.

Tissue specificity: Plasma. Synthesized by the fetal liver and yolk sac.

Developmental stage: Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.

Post-translational modification: Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.Sulfated.

Sequence similarities: Belongs to the ALB/AFP/VDB family.Contains 3 albumin domains.