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Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Rabbit anti-Mouse Mannose Binding Lectin Polyclonal Antibody | anti-MBL antibody

Polyclonal Antibody to Mannose Binding Lectin (MBL)

Gene Names
Mbl2; MBL; L-MBP; MBL-C; MBP-C
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Mannose Binding Lectin; Polyclonal Antibody; Polyclonal Antibody to Mannose Binding Lectin (MBL); anti-MBL antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-MBL antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, Immunoprecipitation (IP).
Application Notes
Western blotting: 0.5-2ug/mL
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Organism Species
Mus musculus (Mouse)
Source
Polyclonal antibody preparation
Cross Reactivity
Mouse
Traits
Liquid
Immunogen
Recombinant MBL (Lys129~Asp244) expressed in E.coli (MBS2030072)
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.

Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Western Blot (WB)

(Western Blot: Sample: Mouse Serum;Primary Ab: 2ug/mL Rabbit Anti-Mouse MBL AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047) )

Western Blot (WB) (Western Blot: Sample: Mouse Serum;Primary Ab: 2ug/mL Rabbit Anti-Mouse MBL AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047) )

Western Blot (WB)

(Western Blot; Sample: Mouse Heart lysate;Primary Ab: 2ug/mL Rabbit Anti-Mouse MBL AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody(Catalog: MBS2086047))

Western Blot (WB) (Western Blot; Sample: Mouse Heart lysate;Primary Ab: 2ug/mL Rabbit Anti-Mouse MBL AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody(Catalog: MBS2086047))

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin-embedded Liver tissue))

Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin-embedded Liver tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
mannose-binding protein C
NCBI Official Synonym Full Names
mannose-binding lectin (protein C) 2
NCBI Official Symbol
Mbl2
NCBI Official Synonym Symbols
MBL; L-MBP; MBL-C; MBP-C
NCBI Protein Information
mannose-binding protein C
UniProt Protein Name
Mannose-binding protein C
Protein Family
UniProt Gene Name
Mbl2
UniProt Synonym Gene Names
MBP-C; RARF/P28A

Uniprot Description

MBL2: Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19 C1|19 25.14 cM

Cellular Component: collagen; extracellular region; extracellular space

Molecular Function: calcium ion binding; calcium-dependent protein binding; carbohydrate binding; galactose binding; mannose binding; protease binding; protein self-association; receptor binding

Biological Process: complement activation, classical pathway; complement activation, lectin pathway; defense response to Gram-positive bacterium; immune system process; innate immune response; killing by host of symbiont cells; negative regulation of viral process; positive regulation of phagocytosis

Research Articles on MBL

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Product Notes

The MBL mbl2 (Catalog #AAA2027422) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Mannose Binding Lectin (MBL) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Mannose Binding Lectin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, Immunoprecipitation (IP). Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the MBL mbl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mannose Binding Lectin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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