Testing Data
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BclI Restriction Enzyme
BclI
Purity
Molecular Biology Grade
Synonyms
BclI; BclI restriction enzyme
Host
Bacillus caldolyticus
Purity/Purification
Molecular Biology Grade
Dilution Buffer
10mM Tris-HCl (pH 7.4 at 25 degree C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
Compatible Ends
BamHI, BglII, Bsp143I, MboI, PsuI
Methylation Effects
BclI cuts neither TGm6ATCA nor TGAThm5CA, but cuts TGATm5CA.
pACYC184
1
Blue/White Cloning Assay
The mix of pUC57/HindIII, pUC57/PstI and pUC57/Eco32I digests was incubated with 10units of enzyme for 16 hours. After religation and transformation 0.3% of white colonies were detected.
Labeled Oligonucleotide (LO) Assay
No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10units of restriction endonuclease for 4 hours.
Lambda
8
Ligation/Recutting Assay
After 50-fold overdigestion (3u/ug DNA x 17 hours) with BclI, more than 90% of the DNA fragments can be ligated at a 5'-termini concentration of 0.1uM. More than 90% of these can be recut.
Overdigestion Assay
No detectable change in the specific fragmentation pattern is observed after 80-fold overdigestion (5u/ug lambda DNA x 16 hours) with BclI (see Star Activity).
Stability During Prolonged Incubation
A minimum of 0.1unit of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 55 degree C.
Storage Buffer
10mM Tris-HCl (pH 7.4 at 25 degree C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
Thermal Inactivation
Only small amounts of enzyme (up to 10units) can be inactivated at 80 degree C for 20min.
Unit Definition
One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA dam-in 1 hour at 55 degree C in 50ul of assay buffer.
Preparation and Storage
-20 degree C
Testing Data
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Testing Data
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Related Product Information for BclI restriction enzyme
5'-T^G A T C A-3'
Product Categories/Family for BclI restriction enzyme
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
BclI
Uniprot Description
Protein family/group databasesRestriction enzymes and methylases database<br/><a href='/database/89'>More..</a>REBASEi242. BclI.
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Product Notes
The BclI (Catalog #AAA638501) is a Restriction Enzyme produced from Bacillus caldolyticus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "BclI, Restriction Enzyme" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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