RSK1, active Active Protein | Rps6ka1 active protein

RSK1, active, Recombinant, Human (Ribosomal S6 Kinase 1, RSK-1, 90kD Ribosomal Protein S6 Kinase 1, p90-RSK 1, p90RSK1, p90S6K, MAP Kinase-activated Protein Kinase 1a, MAPK-activated Protein Kinase 1a, MAPKAP Kinase 1a, MAPKAPK1A, MAPKAPK-1a, Ribosomal Pr

Cat. Num. AAA636895

• Gene Names: Rps6ka1; rsk; Rsk1; p90rsk
• Purity: Highly Purified; ~94% by SDS-PAGE and Coomassie blue staining.
• Synonyms: RSK1; active; Recombinant; Human (Ribosomal S6 Kinase 1; RSK-1; 90kD Ribosomal Protein S6 Kinase 1; p90-RSK 1; p90RSK1; p90S6K; MAP Kinase-activated Protein Kinase 1a; MAPK-activated Protein Kinase 1a; MAPKAP Kinase 1a; MAPKAPK1A; MAPKAPK-1a; Ribosomal Pr; Rps6ka1 active protein

• Host: Sf21 cells
• Purity/Purification: Highly Purified; ~94% by SDS-PAGE and Coomassie blue staining.
• Form/Format: Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride pH 7.5, 0.03% Brij 35, 0.1mM EGTA, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine and 0.1% 2-mercaptoethanol.

• Application Notes: Kinase Assay: Kinase Assay: 0.3-2.5ng phosphorylated 30uM MAPKAP Kinase 2 Substrate Peptide in the assay. Assay background was subtracted from the actual counts to yield the results
• Specific Activity: ~2900U/mg,
• Unit Definition: One unit of RSK1 activity is defined as 1nmol phosphate incorporated into 30uM M2355-09 MAPKAP Kinase 2 Substrate Peptide (KKLNRTLSVA) per minute at 30 degree C with a final ATP concentration of 100uM.
• Preparation and Storage: Aliquot to avoid repeated freezing and thawing and store at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.

Related Product Information for Rps6ka1 active protein

N-terminal His6 tagged recombinant full-length human RSK1 expressed in Sf21 cells. Activated

Product Categories/Family for Rps6ka1 active protein

Molecular Biology; MB-Protein Kinases; Ribosomal

NCBI and Uniprot Product Information

• NCBI GI #: 158636007
• NCBI GeneID: 20111
• NCBI Accession #: NP_033123.2
• NCBI GenBank Nucleotide #: NM_009097.4
• UniProt Accession #: P18653
• Molecular Weight: 84kD
• NCBI Official Full Name: ribosomal protein S6 kinase alpha-1
• NCBI Official Synonym Full Names: ribosomal protein S6 kinase polypeptide 1
• NCBI Official Symbol: Rps6ka1
• NCBI Official Synonym Symbols: rsk; Rsk1; p90rsk
• NCBI Protein Information: ribosomal protein S6 kinase alpha-1; RSK-1; p90S6K; p90RSK1; p90-RSK 1; MAPKAPK-1a; S6K-alpha-1; MAPKAP kinase 1a; ribosomal S6 kinase 1; MAPK-activated protein kinase 1a; 90 kDa ribosomal protein S6 kinase 1; MAP kinase-activated protein kinase 1a; ribosomal protein S6 kinase, 90kD, polypeptide 1; Ribosomal protein S6 kinase, 90kD, 1
• UniProt Protein Name: Ribosomal protein S6 kinase alpha-1
• Protein Family: Ribosomal protein S6 kinase
• UniProt Gene Name: Rps6ka1
• UniProt Synonym Gene Names: Mapkapk1a; Rsk1; S6K-alpha-1; p90-RSK 1; p90RSK1; p90S6K; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a; RSK-1
• UniProt Entry Name: KS6A1_MOUSE

Uniprot Description

p90RSK: an AGC kinase of the RSK family. Phosphorylated and activated by Erk1 and -2 in response to many growth factors, polypeptide hormones and neurotransmitters. Several phosphorylation sites are important for its activation. Possesses two kinase domains connected by a regulator linker region. Phosphorylates a wide range of substrates including ribosomal protein S6. Prominently expressed in brain structures essential for cognitive function and learning.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Translation; Protein kinase, AGC; EC 2.7.11.1; AGC group; RSK family; RSK subfamily

Cellular Component: cytoplasm; spindle; nucleus

Molecular Function: transferase activity; protein serine/threonine kinase activity; caspase inhibitor activity; transferase activity, transferring phosphorus-containing groups; magnesium ion binding; nucleotide binding; protein serine/threonine/tyrosine kinase activity; kinase activity; ribosomal protein S6 kinase activity; transcription factor binding; ATP binding; protein kinase activity

Biological Process: regulation of translation; positive regulation of transcription, DNA-dependent; cellular response to stress; positive regulation of transcription from RNA polymerase II promoter; negative regulation of caspase activity; phosphorylation; protein amino acid phosphorylation; negative regulation of apoptosis