Matriptase Active Protein | ST14 active protein
Matriptase, Recombinant, Human (ST14, SNC19, SNC-19, Epitin, Prostamin, TADG-15, MT-SP1, MTSP1, PRSS14, HAI)
Human matriptase, encoded by the ST14 (suppression of tumorogenicity 14) gene, is also known as tumor associated differentially expressed gene 15 protein/TADG15) and membrane-type serine protease 1/MTSP1 (1). Predicted to have a significant role in tumor biology, matriptase may be a novel target for anticancer therapy (2). However, expressed in most human epithelia, matriptase is also important in several physiological processes (1). For example, it activates prostasin to initiate a protease cascade that is essential for epidermal differentiation (3), and it converts a single chain IGFBPrp1 into the twochain form (4). Matriptase is a type II transmembrane serine protease with a complex modular structure (1). The 855 amino acid (aa) sequence of human matriptase consists of a cytoplasmic tail (aa 155), a transmembrane domain (aa 56-76), and an extracellular portion (aa 77-55). The latter contains the following domains: SEA (aa 86-201), two CUBs (aa 214-334 and 340-47), four LDLRAs (aa 452-486, 487-523, 524-560, and 566-603), and a serine protease (aa 615-855). The physiological activation of the single chain zymogen requires the cleavage at the SEA domain within the ER or Golgi, association with HAI1, which facilitates the transport of the protease to the cell surface, and autocleavage at QARV(615)VGG (1). The activated matriptase is inhibited by HAI1, and the resulting HAI1 complex can be shed from the cell surface (1).
Description:
E. coli-derived recombinant protein corresponding to the sequence Gly596-Val855 of human Matriptase/ST14 protein (NCBI Accession #NP_068813), with an N-terminal Met and 6-His tag. This protein corresponds to the catalytic domain, and is inhibited effectively by rhHAI1 and rhHAI2A. The protein was purified, auto-activated and further purified.
NCBI and Uniprot Product Information
Observed (by SDS-PAGE, reducing conditions): 27kD (major) and minor autoactivation fragments.
NCBI Description
The protein encoded by this gene is an epithelial-derived, integral membrane serine protease. This protease forms a complex with the Kunitz-type serine protease inhibitor, HAI-1, and is found to be activated by sphingosine 1-phosphate. This protease has been shown to cleave and activate hepatocyte growth factor/scattering factor, and urokinase plasminogen activator, which suggest the function of this protease as an epithelial membrane activator for other proteases and latent growth factors. The expression of this protease has been associated with breast, colon, prostate, and ovarian tumors, which implicates its role in cancer invasion, and metastasis. [provided by RefSeq, Jul 2008]
Uniprot Description
ST14: Degrades extracellular matrix. Proposed to play a role in breast cancer invasion and metastasis. Exhibits trypsin-like activity as defined by cleavage of synthetic substrates with Arg or Lys as the P1 site. Defects in ST14 are a cause of ichthyosis autosomal recessive with hypotrichosis (ARIH). ARIH is a skin disorder characterized by congenital ichthyosis associated with the presence of less than the normal amount of hair. Belongs to the peptidase S1 family.
Protein type: EC 3.4.21.109; Protease; Membrane protein, integral
Chromosomal Location of Human Ortholog: 11q24-q25
Cellular Component: extrinsic to plasma membrane; extracellular space; basolateral plasma membrane; integral to plasma membrane; plasma membrane
Molecular Function: serine-type peptidase activity; serine-type endopeptidase activity
Biological Process: keratinocyte differentiation; proteolysis
Disease: Ichthyosis, Congenital, Autosomal Recessive 11
Research Articles on ST14
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Product Notes
The ST14 st14 (Catalog #AAA636644) is an Active Protein produced from Recombinant, E. coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Matriptase, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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