Mouse MECP2 Monoclonal Antibody | anti-MECP2 antibody

Mouse Monoclonal [clone 4B6] (IgG2a,k) to Human MECP2

Cat. Num. AAA245774

• Gene Names: MECP2; RS; RTS; RTT; PPMX; MRX16; MRX79; MRXSL; AUTSX3; MRXS13
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: MECP2; Monoclonal Antibody; Mouse Monoclonal [clone 4B6] (IgG2a; k) to Human MECP2; Anti-MECP2 Antibody (clone 4B6) IHC-plus; AUTSX3; Methyl-CpG-binding protein 2; MRX16; MRX79; MRXSL; RTT; RTS; MeCp-2 protein; RS; MRXS13; PPMX; Human MECP2; anti-MECP2 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 4B6
• Specificity: Human MeCP2
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.4.
• Concentration: 0.42 mg/ml (varies by lot)
• Sequence Length: 486

• Applicable Applications for anti-MECP2 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), WB (1:500 - 1:1000); Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Western blot using tissue lysates, cell lysates and the recombinant protein used as the immunogen.
• Target Species: Human
• Immunogen Description: MECP2 (AAH11612, 81 a.a. ~ 170 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: MECP2 antibody was raised against mECP2 (AAH11612, 81 a.a. ~ 170 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: For long term, store at -20 degree C. Avoid repeat freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-MECP2 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(MECP2 monoclonal antibody clone 4B6 Western blot of MECP2 expression in MCF-7.)

Western Blot (WB)

(MECP2 monoclonal antibody clone 4B6. Western blot of MECP2 expression in NIH/3T3.)

Western Blot (WB)

(MECP2 monoclonal antibody clone 4B6. Western blot of MECP2 expression in rat muscle.)

ELISA (EIA)

(Detection limit for recombinant GST tagged MECP2 is approximately 1 ng/ml as a capture antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 4826830
• NCBI GeneID: 4204
• NCBI Accession #: NP_004983.1
• NCBI GenBank Nucleotide #: NM_004992.3
• UniProt Accession #: P51608; O15233; Q6QHH9; Q7Z384
• Molecular Weight: 53,323 Da
• NCBI Official Full Name: methyl-CpG-binding protein 2 isoform 1
• NCBI Official Synonym Full Names: methyl CpG binding protein 2
• NCBI Official Symbol: MECP2
• NCBI Official Synonym Symbols: RS; RTS; RTT; PPMX; MRX16; MRX79; MRXSL; AUTSX3; MRXS13
• NCBI Protein Information: methyl-CpG-binding protein 2; meCp-2 protein
• UniProt Protein Name: Methyl-CpG-binding protein 2
• Protein Family: Methyl-CpG-binding protein
• UniProt Gene Name: MECP2
• UniProt Synonym Gene Names: MeCp-2 protein; MeCp2
• UniProt Entry Name: MECP2_HUMAN

NCBI Description

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. [provided by RefSeq, Jul 2009]

Uniprot Description

MECP2: Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Interacts with FNBP3. Interacts with CDKL5. Present in all adult somatic tissues tested. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: nucleoplasm; heterochromatin; extracellular space; cytosol; nucleus

Molecular Function: mRNA binding; protein domain specific binding; protein binding; siRNA binding; DNA binding; methyl-CpG binding; double-stranded methylated DNA binding; protein N-terminus binding; chromatin binding; transcription factor binding; transcription corepressor activity; transcription factor activity

Biological Process: catecholamine secretion; genetic imprinting; behavioral fear response; startle response; adult locomotory behavior; positive regulation of transcription, DNA-dependent; chromatin silencing; neurological control of breathing; pathogenesis; negative regulation of transcription from RNA polymerase II promoter; proprioception; sensory perception of pain; neuron maturation; negative regulation of histone methylation; post-embryonic development; phosphatidylcholine metabolic process; negative regulation of histone acetylation; protein localization; synaptogenesis; positive regulation of cell proliferation; dendrite development; visual learning; cerebellum development; histone acetylation; negative regulation of neuron apoptosis; mitochondrial electron transport, ubiquinol to cytochrome c; transcription, DNA-dependent; ventricular system development; glutamine metabolic process; social behavior; respiratory gaseous exchange; cardiolipin metabolic process; histone methylation; positive regulation of synaptogenesis; glucocorticoid metabolic process; regulation of systemic arterial blood pressure by neurological process; long-term memory; inositol metabolic process; response to hypoxia; regulation of excitatory postsynaptic membrane potential; negative regulation of transcription, DNA-dependent

Disease: Rett Syndrome; Mental Retardation, X-linked, Syndromic 13; Autism, Susceptibility To, X-linked 3; Lubs X-linked Mental Retardation Syndrome; Angelman Syndrome; Encephalopathy, Neonatal Severe, Due To Mecp2 Mutations