Rabbit anti-Human GPC6 Polyclonal Antibody | anti-GPC6 antibody

GPC6, CT (GPC6, Glypican-6, Secreted glypican-6)

Cat. Num. AAA646160

• Gene Names: GPC6; OMIMD1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Purified; Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: GPC6; Polyclonal Antibody; CT (GPC6; Glypican-6; Secreted glypican-6); Anti -GPC6; anti-GPC6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Purified; Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-GPC6 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:50-100; Immunohistochemistry: 1:50-100; Flow Cytometry: 1:10-50
• Immunogen: GPC6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 503-536 amino acids from the C-terminal region of human GPC6.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of GPC6 (arrow) using rabbit polyclonal GPC6 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GPC6 gene.)

Immunohistochemistry (IHC)

(GPC6 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GPC6 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(GPC6 Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-GPC6 antibody

The glypicans comprise a family of glycosylphosphatidylinositol-anchored heparan sulfate proteoglycans, and they have been implicated in the control of cell growth and cell division. The glypican encoded by this gene is a putative cell surface coreceptor for growth factors, extracellular matrix proteins, proteases and anti-proteases. [provided by RefSeq].

Product Categories/Family for anti-GPC6 antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 5031719
• NCBI GeneID: 10082
• NCBI Accession #: NP_005699.1
• NCBI GenBank Nucleotide #: NM_005708.3
• UniProt Accession #: Q9Y625; Q96SG5; Q96SG8; Q9H1P4; A8K279
• Molecular Weight: 62,736 Da
• NCBI Official Full Name: glypican-6
• NCBI Official Synonym Full Names: glypican 6
• NCBI Official Symbol: GPC6
• NCBI Official Synonym Symbols: OMIMD1
• NCBI Protein Information: glypican-6; glypican proteoglycan 6
• UniProt Protein Name: Glypican-6
• Protein Family: Glypican
• UniProt Gene Name: GPC6
• UniProt Entry Name: GPC6_HUMAN

NCBI Description

The glypicans comprise a family of glycosylphosphatidylinositol-anchored heparan sulfate proteoglycans, and they have been implicated in the control of cell growth and cell division. The glypican encoded by this gene is a putative cell surface coreceptor for growth factors, extracellular matrix proteins, proteases and anti-proteases. [provided by RefSeq, Jan 2009]

Uniprot Description

Function: Cell surface proteoglycan that bears heparan sulfate. Putative cell surface coreceptor for growth factors, extracellular matrix proteins, proteases and anti-proteases

By similarity. Enhances migration and invasion of cancer cells through WNT5A signaling. Ref.8

Subcellular location: Cell membrane; Lipid-anchor › GPI-anchor; Extracellular side

By similarity. Secreted glypican-6: Secreted › extracellular space

By similarity.

Tissue specificity: Widely expressed. High expression in fetal kidney and lung and lower expressions in fetal liver and brain. In adult tissues, very abundant in ovary, high levels also observed in liver, kidney, small intestine and colon. Not detected in peripheral blood leukocytes. Detected in breast cancer cells (at protein level). Ref.2 Ref.8

Induction: Expression is induced by NFATC2. Ref.8

Involvement in disease: Omodysplasia 1 (OMOD1) [MIM:258315]: A rare autosomal recessive skeletal dysplasia characterized by facial dysmorphism and severe congenital micromelia with shortening and distal tapering of the humeri and femora, to give a club-like appearance. Typical facial features include a prominent forehead, frontal bossing, short nose with a depressed broad bridge, short columella, anteverted nostrils, long philtrum, and small chin.Note: The disease is caused by mutations affecting the gene represented in this entry. Point mutations leading to protein truncation, as well as larger genomic rearrangements resulting in exon deletions, have been found in family segregating omodysplasia type 1. All mutations identified in individuals affected by omodysplasia could lead to the absence of a functional protein, the mutant RNAs being suspected to be nonsense-mediated mRNA decay (NMD) targets. Even if the mRNA escapes NMD and is translated, all mutations are expected to disrupt the three-dimensional protein structure and often to abolish multiple highly conserved cysteine residues. Ref.7

Sequence similarities: Belongs to the glypican family.