Rabbit anti-Human EDNRA Polyclonal Antibody | anti-EDNRA antibody

EDNRA, ID (EDNRA, ETA, ETRA, Endothelin-1 receptor, Endothelin A receptor) (HRP)

Cat. Num. AAA6294427

• Gene Names: EDNRA; ETA; ET-A; ETAR; ETRA; ETA-R; hET-AR
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: EDNRA; Polyclonal Antibody; ID (EDNRA; ETA; ETRA; Endothelin-1 receptor; Endothelin A receptor) (HRP); Endothelin A receptor; anti-EDNRA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-EDNRA antibody: Western Blot (WB), ELISA (EIA), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:50-100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: EDNRA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 132~162 amino acids from the Center region of human EDNRA.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Western Blot (WB)

(Western blot analysis of EDNRA antibody (Center) in CEM cell line lysates (35ug/lane). EDNRA (arrow) was detected using the purified Pab.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of EDNRA Antibody (Center) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). )

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of CEM cells using EDNRA Antibody (Center) (green histogram) compared to a negative control cell (blue histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-EDNRA antibody

Antibodies; Endotoxin

References

Cardillo, C., Arthritis Rheum. 60 (6), 1840-1844 (2009). Hayzer, D.J., Am. J. Med. Sci. 304 (4), 231-238 (1992).

NCBI and Uniprot Product Information

• NCBI GI #: 260593650
• NCBI GeneID: 1909
• NCBI Accession #: NP_001159527.1
• NCBI GenBank Nucleotide #: NM_001166055.1
• UniProt Accession #: P25101; O43441; Q16432; Q16433; Q8TBH2; B2R723; B4E2V6; B7Z9G6; D3DP03; E7ER36; AAB24923
• Molecular Weight: 48,722 Da
• NCBI Official Full Name: endothelin-1 receptor isoform b
• NCBI Official Synonym Full Names: endothelin receptor type A
• NCBI Official Symbol: EDNRA
• NCBI Official Synonym Symbols: ETA; ET-A; ETAR; ETRA; ETA-R; hET-AR
• NCBI Protein Information: endothelin-1 receptor; G protein-coupled receptor; endothelin receptor subtype A; endothelin-1-specific receptor
• UniProt Protein Name: Endothelin-1 receptor
• Protein Family: Endothelin-1 receptor
• UniProt Gene Name: EDNRA
• UniProt Synonym Gene Names: ETA; ETRA; ET-A; ETA-R; hET-AR
• UniProt Entry Name: EDNRA_HUMAN

NCBI Description

This gene encodes the receptor for endothelin-1, a peptide that plays a role in potent and long-lasting vasoconstriction. This receptor associates with guanine-nucleotide-binding (G) proteins, and this coupling activates a phosphatidylinositol-calcium second messenger system. Polymorphisms in this gene have been linked to migraine headache resistance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]

Uniprot Description

EDNRA: Receptor for endothelin-1. Mediates its action by association with G proteins that activate a phosphatidylinositol- calcium second messenger system. The rank order of binding affinities for ET-A is: ET1 > ET2 >> ET3. Belongs to the G-protein coupled receptor 1 family. Endothelin receptor subfamily. EDNRA sub-subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral; Motility/polarity/chemotaxis; GPCR, family 1

Chromosomal Location of Human Ortholog: 4q31.22

Cellular Component: nuclear membrane; integral to plasma membrane; T-tubule; plasma membrane; lipid raft

Molecular Function: endothelin receptor activity; protein binding; phosphoinositide phospholipase C activity

Biological Process: positive regulation of odontogenesis; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); neural crest cell development; metabolic process; heart development; response to morphine; smooth muscle cell proliferation; response to lipopolysaccharide; glucose transport; signal transduction; sensory perception of pain; enteric nervous system development; regulation of epithelial cell proliferation; Rho protein signal transduction; histamine secretion; elevation of cytosolic calcium ion concentration; negative regulation of cAMP biosynthetic process; smooth muscle contraction; fibroblast proliferation; regulation of blood pressure; protein kinase C activation; positive regulation of cell proliferation; artery smooth muscle contraction; penile erection; aging; vasoconstriction; in utero embryonic development; glomerular filtration; adenylate cyclase activation; respiratory gaseous exchange; patterning of blood vessels; cell proliferation; G-protein coupled receptor protein signaling pathway; phospholipase C activation; maternal process involved in parturition; response to hypoxia; positive regulation of protein amino acid phosphorylation; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of inflammatory response; negative regulation of apoptosis

Disease: Mandibulofacial Dysostosis With Alopecia; Migraine With Or Without Aura, Susceptibility To, 1