Typical Testing Data/Standard Curve (for reference only)
Human Aflatoxin B1 aldehyde reductase member 2 (AKR7A2) ELISA Kit | AKR7A2 elisa kit
Human Aflatoxin B1 aldehyde reductase member 2 (AKR7A2) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: AKR7A2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-AKR7A2 antibody and an AKR7A2-HRP conjugate. The assay sample and buffer are incubated together with AKR7A2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AKR7A2 concentration since AKR7A2 from samples and AKR7A2-HRP conjugate compete for the anti-AKR7A2 antibody binding site. Since the number of sites is limited, as more sites are occupied by AKR7A2 from the sample, fewer sites are left to bind AKR7A2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AKR7A2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the aldo/keto reductase (AKR) superfamily and AKR7 family, which are involved in the detoxification of aldehydes and ketones. The AKR7 family consists of 3 genes that are present in a cluster on the p arm of chromosome 1. This protein, thought to be localized in the golgi, catalyzes the NADPH-dependent reduction of succinic semialdehyde to the endogenous neuromodulator, gamma-hydroxybutyrate. It may also function as a detoxication enzyme in the reduction of aflatoxin B1 and 2-carboxybenzaldehyde. [provided by RefSeq, Oct 2011]
Uniprot Description
AKR7A2: Catalyzes the NADPH-dependent reduction of succinic semialdehyde to gamma-hydroxybutyrate. May have an important role in producing the neuromodulator gamma-hydroxybutyrate (GHB). Has broad substrate specificity. Has NADPH-dependent aldehyde reductase activity towards 2-carboxybenzaldehyde, 2- nitrobenzaldehyde and pyridine-2-aldehyde (in vitro). Can reduce 1,2-naphthoquinone and 9,10-phenanthrenequinone (in vitro). Can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the non-binding AFB1 dialcohol. May be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen. Belongs to the aldo/keto reductase family. Aldo/keto reductase 2 subfamily.
Protein type: Oxidoreductase; EC 1.1.1.n11
Chromosomal Location of Human Ortholog: 1p36.13
Cellular Component: Golgi apparatus; mitochondrion; cytosol
Molecular Function: electron carrier activity; aldehyde reductase activity; phenanthrene-9,10-epoxide hydrolase activity
Biological Process: xenobiotic metabolic process; carbohydrate metabolic process; aldehyde metabolic process
Research Articles on AKR7A2
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Product Notes
The Human AKR7A2 akr7a2 (Catalog #AAA7238070) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7238070 ELISA Kit recognizes Human AKR7A2. It is sometimes possible for the material contained within the vial of "Aflatoxin B1 aldehyde reductase member 2 (AKR7A2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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