Rabbit IRS-2 Polyclonal Antibody | anti-IRS2 antibody

Anti-IRS-2 (Insulin Receptor Substrate 2)

Cat. Num. AAA629200

• Gene Names: IRS2; IRS-2
• Reactivity: Human; Mouse; Rat
• Applications: Immunoprecipitation, Western Blot
• Purity: Affinity Purified
• Synonyms: IRS-2; Polyclonal Antibody; Anti-IRS-2 (Insulin Receptor Substrate 2); Insulin Receptor Substrate 2; anti-IRS2 antibody

• Host: Rabbit
• Reactivity: Human; Mouse; Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse IRS-2. Species Crossreactivity: rat and human.
• Purity/Purification: Affinity Purified
• Form/Format: Supplied as a liquid in 0.1M Tris, pH7.4, 0.1% BSA.

• Applicable Applications for anti-IRS2 antibody: Western Blot; Immunoprecipitation
• Application Notes: Suitable for use in Immunoprecipitation and Western Blot.; Recommended Dilutions: Immunoprecipitation: 2-10ug or ul using 500ug lysate (control cells 3T3/A31 RIPA lysate).; Western Blot: 1:500-1:2000 or 1-5 ug/ml (chemiluminescence)
• Immunogen: Mouse IRS-2, 976-1094-GST fusion protein.
• Source Information: Mouse
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.; Shipping Temperature: Blue Ice

Related Product Information for anti-IRS2 antibody

Insulin receptor substrate (IRS) proteins play a pivotal role in signal transduction by the receptors for insulin, insulin-like growth factor 1 (IGF-1), and a growing number of cytokines and integrins. Four IRS proteins (IRS-1, IRS-2, IRS-3 and IRS-4) have beenidentified and characterized. Recently, two more signaling proteins, IRS5/DOK4 and IRS6/DOK5, have been identified as potentially new members of the IRS family. IRSs are tyrosine-phosphorylated following insulin or IGF-1 stimulation and form signaling complexes at the receptor with Src homology 2 domain-containing proteins, including the p85 regulatory subunit of phosphatidylinositol 3-kinase, Grb2, Nck, and Shc. IRS family of proteins share a number of structural and functional characteristics, including an N-terminal pleckstrin homology (PH) and a phosphotyrosine binding domain (PTB), followed by a large C-terminal region containing multiple sites of tyrosine phosphorylation, which serve as docking sites for Src homology 2 domain-containing signaling proteins. Despite the overall similarity, the IRS proteins differin their subcellular distribution and tissue/developmental expression pattern. Each IRS is phosphorylated on a set of specific tyrosine residues, leading to its binding to the Src homology 2 domains of distinct signaling proteinsand therefore, activation of specific tyrosine residues, leading to its binding to the Src homology 2 domains of distinct signaling proteins and therefore, activation of specific signaling cascades. Disruption of each individual IRS gene causes distinct phenotypes in mice, further indicating that the four IRS proteins play different roles in the regulation of the pleiotropic effects of insulin, IGF-1, and other growth factors.

Product Categories/Family for anti-IRS2 antibody

Abs to Protein Kinase Substrates

NCBI and Uniprot Product Information

• NCBI GI #: 38683860
• NCBI GeneID: 8660
• NCBI Accession #: NP_003740.2
• NCBI GenBank Nucleotide #: NM_003749.2
• UniProt Accession #: Q96RG4; Q8TF73; Q96RG5; Q9P084; Q9UP29; Q9Y4H2
• Molecular Weight: 137,319 Da
• NCBI Official Full Name: insulin receptor substrate 2
• NCBI Official Synonym Full Names: insulin receptor substrate 2
• NCBI Official Symbol: IRS2
• NCBI Official Synonym Symbols: IRS-2
• NCBI Protein Information: insulin receptor substrate 2; OTTHUMP00000018696
• UniProt Protein Name: Insulin receptor substrate 2 insertion mutant
• Protein Family: Insulin receptor substrate
• UniProt Gene Name: IRS2
• UniProt Entry Name: Q96RG4_HUMAN