Mouse IRS-1 Monoclonal Antibody | anti-IRS1 antibody

IRS-1, phosphorylated (Ser616) (Insulin Receptor Substrate 1, IRS1)

Cat. Num. AAA609061

• Gene Names: IRS1; HIRS-1
• Applications: ELISA
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: IRS-1; Monoclonal Antibody; phosphorylated (Ser616) (Insulin Receptor Substrate 1; IRS1); Anti -IRS-1; anti-IRS1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 10g79
• Specificity: Recognizes human Phospho-IRS (Ser616).
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.5% BSA, 0.01% thimerosal, 30% glycerol.

• Applicable Applications for anti-IRS1 antibody: ELISA (EL/EIA)
• Application Notes: Suitable for use in Indirect ELISA.; Dilution: Indirect ELISA: 1-2ug/ml
• Immunogen: Synthetic peptide corresponding to IRS-1, phosphorylated at Ser616 site.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

ELISA (EIA)

(Indirect ELISA Serial dilution of the antibody starting with 5ug/well was done to check for the affinity. 1 μg of coated antigen can be sensitively detected by anti-human IRS Ser 616-Phospho antibody used at 300 pg/well concentration.)

Related Product Information for anti-IRS1 antibody

Insulin receptor substrates (IRS) subtypes are responsible for several insulin related activities, such as glucose homeostasis, cell growth, cell transformation, apoptosis and insulin signal transduction. The balance between Ser/Thr phosphorylation of IRS has been demonstrated to be an important regulator of insulin signaling. IRS1 has also been shown to be constitutively activated in cancers such as breast cancer, Wilm's tumors, and adrenal cortical carcinomas. Does not recognize IRS peptide NOT phosphorylated at Ser616 and will not recognize IRS peptide phosphorylated at Tyr183. Also does not react with riboflavin carrier protein which is known to have multiple phosphate groups.

Product Categories/Family for anti-IRS1 antibody

Antibodies; Abs to Protein Kinase Substrates

NCBI and Uniprot Product Information

• NCBI GI #: 5031805
• NCBI GeneID: 3667
• NCBI Accession #: NP_005535.1
• NCBI GenBank Nucleotide #: NM_005544.2
• UniProt Accession #: P35568
• Molecular Weight: 131,591 Da
• NCBI Official Full Name: insulin receptor substrate 1
• NCBI Official Synonym Full Names: insulin receptor substrate 1
• NCBI Official Symbol: IRS1
• NCBI Official Synonym Symbols: HIRS-1
• NCBI Protein Information: insulin receptor substrate 1; IRS-1; OTTHUMP00000164234
• UniProt Protein Name: Insulin receptor substrate 1
• Protein Family: Isoleucine--tRNA ligase
• UniProt Gene Name: IRS1
• UniProt Entry Name: IRS1_HUMAN

NCBI Description

This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq]

Uniprot Description

IRS1: an adaptor protein that is one of the major substrates of the insulin receptor kinase. Contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain-containing proteins including phosphatidylinositol 3-kinase p85 subunit and GRB-2. Phosphorylated on ser/thr residues by kinases including JNK and IKK.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 2q36

Cellular Component: intracellular membrane-bound organelle; cytoplasm; plasma membrane; caveola; nucleus; cytosol

Molecular Function: insulin-like growth factor receptor binding; protein binding; signal transducer activity; protein kinase C binding; phosphoinositide 3-kinase binding; SH2 domain binding; transmembrane receptor protein tyrosine kinase adaptor protein activity; insulin receptor binding

Biological Process: response to peptide hormone stimulus; phosphoinositide 3-kinase cascade; nerve growth factor receptor signaling pathway; positive regulation of glycogen biosynthetic process; negative regulation of insulin receptor signaling pathway; glucose homeostasis; signal transduction; response to insulin stimulus; negative regulation of insulin secretion; positive regulation of glucose import; mammary gland development; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; protein kinase B signaling cascade; epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; positive regulation of phosphoinositide 3-kinase activity; positive regulation of fatty acid beta-oxidation; insulin-like growth factor receptor signaling pathway; cellular response to insulin stimulus; regulation of gene expression; insulin receptor signaling pathway; innate immune response; positive regulation of insulin receptor signaling pathway; lipid catabolic process; positive regulation of cell migration

Disease: Diabetes Mellitus, Noninsulin-dependent