Rabbit IRS-2 (pS731) Antibody | anti-IRS-2 (pS731) antibody

Rabbit anti Phospho- IRS-2 (pSer 731) antibody

Cat. Num. AAA462062

• Gene Names: IRS2; IRS-2
• Reactivity: Human, Rat, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.
• Synonyms: IRS-2 (pS731); Rabbit anti Phospho- IRS-2 (pSer 731) antibody; Rabbit anti-IRS-2 (p Ser731); Anti-phospho-IRS-2 (pSer731); Insulin-receptor substrate 2 (IRS-2).; anti-IRS-2 (pS731) antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Specificity: This antibody recognizes ~180 kDa of human IRS-2 (pSer731). It also reacts with mouse and rat. The other species are not tested.
• Purity/Purification: The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.
• Form/Format: This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
• Concentration: Size: 100 ug/200 ul (varies by lot)

• Applicable Applications for anti-IRS-2 (pS731) antibody: ELISA, Western Blot, Immunohistochemistry
• Application Notes: Western Blot: 0.1-1 ug/ml; ELISA: 0.01-0.1 ug/ml; Immunoprecipitation: 2-5 ug/ml; Immunohistochemistry: 0.5-2 ug/ml
• Antigen Preparation: A synthetic peptide corresponding to the epitope ASSPA with a single phosphorylation site Ser 731 of human IRS-2.
• Positive Control: 3T3 cell lysate
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Testing Data

Related Product Information for anti-IRS-2 (pS731) antibody

Insulin Receptor Substrate-2 (IRS-2), a 180kDa cytoplasmic docking protein, is one of the major endogenous substrates of the insulin receptor kinase. The insulin receptor mediates insulin action through the phosphorylation of substrate proteins. The major substrates of the insulin receptor kinase are the insulin receptor substrate (IRS) family of proteins, IRS 1, IRS 2, IRS 3, and IRS 4. IRS 2, a signaling molecule that mediates effects of insulin, Insulin-like growth factor 1 and other cytokines by acting as a molecular adaptor between diverse receptor kinases and downstream effectors. IRS proteins contain more than 70 potential Ser / Thr phosphorylation sites for kinases like PKA (cAMP-dependent protein kinase), PKC, and MAPK. The phosphorylation of Ser / Thr residues of IRS proteins has a dual function, serving either for a positive or negative modulation of insulin signal transduction.

References

Li, Y. et al. (2004) J. Biol. Chem. 279, 45304-45307.

NCBI and Uniprot Product Information

• NCBI GI #: 38683860
• NCBI GeneID: 8660
• NCBI Accession #: NP_003740.2
• NCBI GenBank Nucleotide #: NM_003749.2
• UniProt Accession #: Q96RG4; Q8TF73; Q96RG5; Q9P084; Q9UP29; Q9Y4H2
• Molecular Weight: 180 kDa
• NCBI Official Full Name: insulin receptor substrate 2
• NCBI Official Synonym Full Names: insulin receptor substrate 2
• NCBI Official Symbol: IRS2
• NCBI Official Synonym Symbols: IRS-2
• NCBI Protein Information: insulin receptor substrate 2; OTTHUMP00000018696
• UniProt Protein Name: Insulin receptor substrate 2 insertion mutant
• UniProt Gene Name: IRS2
• UniProt Entry Name: Q96RG4_HUMAN

NCBI Description

This gene encodes the insulin receptor substrate 2, a cytoplasmic signaling molecule that mediates effects of insulin, insulin-like growth factor 1, and other cytokines by acting as a molecular adaptor between diverse receptor tyrosine kinases and downstream effectors. The product of this gene is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation, as well as by an interleukin 4 receptor-associated kinase in response to IL4 treatment. [provided by RefSeq]

Uniprot Description

Sequence similarities: Contains 1 PH domain. RuleBase RU003350