Rabbit anti-Human IRS-2 Polyclonal Antibody | anti-IRS2 antibody

IRS-2, phosphorylated (Tyr978) (Insulin Receptor Substrate 2, IRS2)

Cat. Num. AAA631034

• Gene Names: IRS2; IRS-2
• Reactivity: Human
• Applications: ELISA, Gel Super Shift Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: IRS-2; Polyclonal Antibody; phosphorylated (Tyr978) (Insulin Receptor Substrate 2; IRS2); Anti -IRS-2; anti-IRS2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human IRS2 when phosphorylated at Tyr978.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-IRS2 antibody: ELISA (EL/EIA), Gel Shift Assay (GS/EMSA)
• Application Notes: Suitable for use in ELISA and Dot Blot.; Dilution: ELISA: 1:1,000; Dot Blot: 1:500
• Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Tyr978 of human IRS2 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of anti-Phospho-IRS2-pY978 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Related Product Information for anti-IRS2 antibody

Insulin receptor substrate 2, a cytoplasmic signaling molecule that mediates effects of insulin, insulin-like growth factor 1, and other cytokines by acting as a molecular adaptor between diverse receptor tyrosine kinases and downstream effectors. This protein is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation, as well as by an interleukin 4 receptor-associated kinase in response to IL4 treatment.

Product Categories/Family for anti-IRS2 antibody

Antibodies; Abs to Protein Kinase Substrates

NCBI and Uniprot Product Information

• NCBI GI #: 38683860
• NCBI GeneID: 8660
• NCBI Accession #: NP_003740.2
• NCBI GenBank Nucleotide #: NM_003749.2
• UniProt Accession #: Q96RG4; Q8TF73; Q96RG5; Q9P084; Q9UP29; Q9Y4H2
• Molecular Weight: 137,319 Da
• NCBI Official Full Name: insulin receptor substrate 2
• NCBI Official Synonym Full Names: insulin receptor substrate 2
• NCBI Official Symbol: IRS2
• NCBI Official Synonym Symbols: IRS-2
• NCBI Protein Information: insulin receptor substrate 2; OTTHUMP00000018696
• UniProt Protein Name: Insulin receptor substrate 2 insertion mutant
• Protein Family: Insulin receptor substrate
• UniProt Gene Name: IRS2
• UniProt Entry Name: Q96RG4_HUMAN

NCBI Description

This gene encodes the insulin receptor substrate 2, a cytoplasmic signaling molecule that mediates effects of insulin, insulin-like growth factor 1, and other cytokines by acting as a molecular adaptor between diverse receptor tyrosine kinases and downstream effectors. The product of this gene is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation, as well as by an interleukin 4 receptor-associated kinase in response to IL4 treatment. [provided by RefSeq]

Uniprot Description

IRS2: May mediate the control of various cellular processes by insulin. Interacts with PHIP.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 13q34

Cellular Component: plasma membrane; cytosol

Molecular Function: protein domain specific binding; protein binding; signal transducer activity; phosphoinositide 3-kinase binding; protein phosphatase binding; protein kinase binding; insulin receptor binding

Biological Process: negative regulation of kinase activity; regulation of lipid metabolic process; nerve growth factor receptor signaling pathway; positive regulation of glycogen biosynthetic process; lipid homeostasis; signal transduction; positive regulation of glucose import; mammary gland development; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; response to glucose stimulus; positive regulation of B cell proliferation; epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; glucose metabolic process; positive regulation of insulin secretion; positive regulation of fatty acid beta-oxidation; cell proliferation; cellular response to insulin stimulus; insulin receptor signaling pathway; innate immune response; brain development; negative regulation of B cell apoptosis; positive regulation of cell migration

Disease: Diabetes Mellitus, Noninsulin-dependent