Rabbit Src Polyclonal Antibody | anti-Src antibody

Src, phosphorylated (Tyr529) (pp60Src, pp60c-Src)

Cat. Num. AAA626711

• Gene Names: Src; AW259666; pp60c-src
• Reactivity: Chicken, Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Src; Polyclonal Antibody; phosphorylated (Tyr529) (pp60Src; pp60c-Src); Anti -Src; anti-Src antibody

• Host: Rabbit
• Reactivity: Chicken, Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes human Src when phosphorylated at Tyr418. Species crossreactivity
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.

• Applicable Applications for anti-Src antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1:1000; Optimal dilution to be determined by the researcher.
• Immunogen: Synthetic phosphopeptide corresponding to human Src that contains tyrosine 529 (tyrosine 530 including the initiating methionine).
• Positive Control: Chick Embryo Fibroblast (CEF) cells expressing human wild-type Src protein.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Src antibody

Src (also known as pp60src) is a non-receptor tyrosine kinase involved in signal transduction in many biological systems and implicated in the development of human tumors. Tyrosine 529 is located near the carboxyl terminus of Src and acts as a negative regulator, in that Src is held in the inactive form through an intramolecular interaction between the SH2 domain and the carboxyl terminus when tyrosine 529 is phosphorylated by Csk. This conformation blocks phosphorylation of the catalytic domain residue (tyrosine 418 in the human sequence), thereby preventing Src activation. When tyrosine 529 is dephosphorylated, tyrosine 418 can be maximally phosphorylated leading to full activation.

Product Categories/Family for anti-Src antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 62635494
• NCBI GeneID: 20779
• UniProt Accession #: P05480; Q2M4I4
• Molecular Weight: 60,645 Da
• NCBI Official Full Name: Src
• NCBI Official Synonym Full Names: Rous sarcoma oncogene
• NCBI Official Symbol: Src
• NCBI Official Synonym Symbols: AW259666; pp60c-src
• NCBI Protein Information: neuronal proto-oncogene tyrosine-protein kinase Src; p60-Src; proto-oncogene c-Src
• UniProt Protein Name: Neuronal proto-oncogene tyrosine-protein kinase Src
• Protein Family: Src-like-adapter
• UniProt Gene Name: Src
• UniProt Synonym Gene Names: p60-Src
• UniProt Entry Name: SRC_MOUSE

Uniprot Description

Src: proto-oncogenic cytoplasmic tyrosine kinase of the SRC family. Highly expressed in certain fully differentiated cells such as neurons, platelets and macrophages. Phosphorylation of an activation loop tyrosine activates the enzyme; phosphorylation of a tyrosine in the C-terminus by Csk inhibits the enzyme. Two alternatively spliced isoforms have been described.

Protein type: Oncoprotein; Kinase, protein; EC 2.7.10.2; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); TK group; Src family

Cellular Component: extrinsic to internal side of plasma membrane; cytoskeleton; membrane; mitochondrion; perinuclear region of cytoplasm; mitochondrial inner membrane; cytoplasm; plasma membrane; actin filament; intracellular; caveola; nucleus

Molecular Function: transferase activity; protein domain specific binding; protein binding; ephrin receptor binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; non-membrane spanning protein tyrosine kinase activity; kinase activity; ATP binding; hormone receptor binding; protein kinase activity

Biological Process: oogenesis; epidermal growth factor receptor signaling pathway; regulation of estrogen receptor signaling pathway; cell migration; peptidyl-tyrosine phosphorylation; progesterone receptor signaling pathway; regulation of cell cycle; immune system process; platelet-derived growth factor receptor signaling pathway; uterus development; cell cycle; protein amino acid phosphorylation; regulation of cell proliferation; regulation of protein binding; forebrain development; innate immune response; cell adhesion; phosphorylation; bone resorption