Goat PRMT6 Polyclonal Antibody | anti-PRMT6 antibody

Goat anti-PRMT6 Antibody

Cat. Num. AAA422909

• Gene Names: PRMT6; HRMT1L6
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Pig
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: PRMT6; Polyclonal Antibody; Goat anti-PRMT6 Antibody; protein arginine methyltransferase 6; FLJ10559; FLJ51477; HRMT1L6; HMT1 hnRNP methyltransferase-like 6; OTTHUMP00000012633; heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 6; histone-arginine N-methyltransferase PRMT6; protei; FLJ10559 antibody; FLJ51477 antibody; heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 6 antibody; histone-arginine N-methyltransferase PRMT6 antibody; HMT1 hnRNP methyltransferase-like 6 antibody; HRMT1L6 antibody; OTTHUMP00000012633 antibody; PRMT6 antibody; protein arginine methyltransferase 6 antibody; protein arginine N-methyltransferase 6 antibody; anti-PRMT6 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Pig
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: ERDQLYYECYSD
• Sequence Length: 375

• Applicable Applications for anti-PRMT6 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 128000.; Western Blot: Approx 45kDa band observed in lysates of cell line MCF7 (calculated MW of 41.9kDa according to NP_060607.2). Recommended concentration: 0.5-2ug/ml.
• Immunogen: Peptide with sequence ERDQLYYECYSD, from the internal region of the protein sequence according to NP_060607.2.
• Epitope: Internal region
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.5ug/ml) staining of MCF7 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 227908867
• NCBI GeneID: 55170
• NCBI Accession #: NP_060607.2
• NCBI GenBank Nucleotide #: NM_018137.2
• Molecular Weight: 32,509 Da
• NCBI Official Full Name: protein arginine N-methyltransferase 6
• NCBI Official Synonym Full Names: protein arginine methyltransferase 6
• NCBI Official Symbol: PRMT6
• NCBI Official Synonym Symbols: HRMT1L6
• NCBI Protein Information: protein arginine N-methyltransferase 6
• UniProt Protein Name: Protein arginine N-methyltransferase 6
• Protein Family: Protein arginine N-methyltransferase
• UniProt Gene Name: PRMT6
• UniProt Synonym Gene Names: HRMT1L6
• UniProt Entry Name: ANM6_HUMAN

NCBI Description

The protein encoded by this gene belongs to the arginine N-methyltransferase family, which catalyze the sequential transfer of methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins, to form methylated arginine derivatives and S-adenosyl-L-homocysteine. This protein can catalyze both, the formation of omega-N monomethylarginine and asymmetrical dimethylarginine, with a strong preference for the latter. It specifically mediates the asymmetric dimethylation of Arg2 of histone H3, and the methylated form represents a specific tag for epigenetic transcriptional repression. This protein also forms a complex with, and methylates DNA polymerase beta, resulting in stimulation of polymerase activity by enhancing DNA binding and processivity. [provided by RefSeq, Sep 2011]

Uniprot Description

PRMT6: Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA), with a strong preference for the formation of aDMA. Preferentially methylates arginyl residues present in a glycine and arginine-rich domain and displays preference for monomethylated substrates. Specifically mediates the asymmetric dimethylation of histone H3 'Arg-2' to form H3R2me2a. H3R2me2a represents a specific tag for epigenetic transcriptional repression and is mutually exclusive with methylation on histone H3 'Lys-4' (H3K4me2 and H3K4me3). It thereby acts as a transcription corepressor of various genes such as HOXA2. Also methylates histone H2A and H4 'Arg-3' (H2AR3me and H4R3me, respectively). Acts as a regulator of DNA base excision during DNA repair by mediating the methylation of DNA polymerase beta (POLB), leading to stimulate the polymerase activity by enhancing DNA binding and processivity. Methylates HMGA1. May play a role in innate immunity against HIV-1 in case of infection by methylating and impairing the function of various HIV-1 proteins such as Tat, Rev and Nucleocapsid protein p7 (NC). Belongs to the protein arginine N-methyltransferase family. PRMT6 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.1.1.125; Methyltransferase, protein arginine; Methyltransferase

Chromosomal Location of Human Ortholog: 1p13.3

Cellular Component: cytosol; nucleoplasm; nucleus

Molecular Function: chromatin binding; histone binding; histone methyltransferase activity; histone-arginine N-methyltransferase activity; protein binding; protein-arginine N-methyltransferase activity; protein-arginine omega-N asymmetric methyltransferase activity; protein-arginine omega-N monomethyltransferase activity

Biological Process: base-excision repair; cell growth; histone methylation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; peptidyl-arginine methylation, to asymmetrical-dimethyl arginine; transcription, DNA-dependent; viral reproduction