Rabbit MAP Kinase, p38 Polyclonal Antibody

MAP Kinase, p38 (MAPK14, Mitogen-activated Protein Kinase 14, CSBP)

Cat. Num. AAA623786

• Reactivity: Guinea Pig, Human, Monkey, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography and immunoaffinity chromatography.
• Synonyms: MAP Kinase; p38; Polyclonal Antibody; p38 (MAPK14; Mitogen-activated Protein Kinase 14; CSBP); Anti -MAP Kinase; anti-MAP Kinase; p38 antibody

• Host: Rabbit
• Reactivity: Guinea Pig, Human, Monkey, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes endogenous levels of total human p38 alpha, beta, or gamma MAP kinase protein (43kD). Does not recognize p38 delta, JNK/SAPK or p44/42 MAP kinase. Species Crossreactivity: mouse, monkey, rat, monkey and guinea pig. Expected to cross-react with chicken based on sequence homology.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography and immunoaffinity chromatography.
• Form/Format: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

• Applicable Applications for anti-MAP Kinase, p38 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Suitable for use in Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistr (paraffin sections) and Western Blot.; Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.; Immunohistochemistry (Paraffin): 1:200. Requires antigen retrieval using ctirate method.; Immunocytochemistry/Immunofluorescence: 1:50; Flow Cytometry: 1:50
• Immunogen: Synthetic peptide (KLH coupled) derived from the sequence of human p38 MAP kinase.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of extracts from NIH/3T3 cells, untreated or UV-treated for the indicated times, using Phospho-p38 MAP Kinase (Thr180/Tyr182) (upper) or MBS623786 (lower).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear and cytoplasmic localization, using MBS623786)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Jurkat cells, using MBS623786(blue) compared to a nonspecific negative control antibody (red).)

Related Product Information for anti-MAP Kinase, p38 antibody

P38 is the newest member of the MAP kinase family. It is a reactivating kinase. It is activated in response to inflammatory cytokines, endotoxins, and osmotic stress. p38 MAP kinase (MAPK), also called RK (3) or CSBP (2), is the mammalian homologue of the yeast HOG kinase and participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAP kinase, alpha, beta, gamma and delta, have been identified so far. Like the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides (LPS), UV light and growth factors (1-5). MKK3 and SEK activate p38 MAP kinase by phosphorylation at threonine 180 and tyrosine 182. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6) and MEF2 (5-8).

Product Categories/Family for anti-MAP Kinase, p38 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 529040
• NCBI Official Full Name: MAP kinase