Rabbit MAP Kinase Kinase 3,6 Polyclonal Antibody | anti-MAP2K3 antibody

MAP Kinase Kinase 3,6, phosphorylated (Ser189/207) (MKK3,6, MAP Kinase Kinase 3, MKK3, MAP Kinase Kinase 6, MKK6)

Cat. Num. AAA619204

• Gene Names: MAP2K3; MEK3; MKK3; MAPKK3; PRKMK3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: MAP Kinase Kinase 3; 6; Polyclonal Antibody; phosphorylated (Ser189/207) (MKK3; MKK3; MAP Kinase Kinase 6; MKK6); Anti -MAP Kinase Kinase 3; anti-MAP2K3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recongizes endogenous levels of human MKK3/MKK6 only when phosphorylated at Ser189/207. Does not recognize the corresponding phosphorylated residues of MEK1, MEK2 or MKK4/SEK1. Species Crossreactivity: Mouse and rat.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added. Also available without BSA & glycerol. See M2363-15C.

• Applicable Applications for anti-MAP2K3 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunoprecipitation.; Dilution: Western Blot: 1:1000; Immunoprecipitation: 1:250
• Immunogen: Synthetic phosphopeptide corresponding to residues around Ser189/207 of human MKK3 (KLH coupled).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from NIH/3T3 cells, untreated or anisomycin-treated for the indicated times, using MBS619204 MAP Kinase Kinase 3,6, phosphorylated (Ser189/207) (MKK3,6) (upper) or M2363-10 MAP Kinase Kinase 3 (MKK3) (lower).)

Related Product Information for anti-MAP2K3 antibody

MKK3 and MKK6 are two closely related dual-specificity protein kinases that activate p38 MAP kinase (1-5). MKK3 and MKK6 both phosphorylate and activate p38 MAP kinase at its activation site, Thr-Gly-Tyr. They do not phosphorylate or activate the two other MAP kinases, Erk1/2 or SAPK/JNK. Phosphorylation of p38 MAP kinase dramatically stimulates the ability of p38 MAP kinase to phosphorylate protein substrates such as ATF-2 and Elk-1. MKK3 and MKK6 are both activated by different forms of cellular stress and inflammatory cytokines (4,5). Activation of MKK3 and MKK6 occurs through phosphorylation of serine and threonine residues at sites Ser189 and Thr193 for MKK3 (2), and Ser207 and Thr211 for MKK6 (4,5).

Product Categories/Family for anti-MAP2K3 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 685174
• NCBI GeneID: 5606
• UniProt Accession #: P46734; Q99441; Q9UE71; Q9UE72; B3KSK7; Q53EZ9; Q6FHG1; Q6FI23
• Molecular Weight: 39,318 Da
• NCBI Official Full Name: MAP kinase kinase 3
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 3
• NCBI Official Symbol: MAP2K3
• NCBI Official Synonym Symbols: MEK3; MKK3; MAPKK3; PRKMK3
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 3; MEK 3; MAPKK 3; MAPK/ERK kinase 3; OTTHUMP00000166043; OTTHUMP00000166044; MAP kinase kinase 3
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 3
• Protein Family: Dual specificity mitogen-activated protein kinase kinase
• UniProt Gene Name: MAP2K3
• UniProt Synonym Gene Names: MEK3; MKK3; PRKMK3
• UniProt Entry Name: MP2K3_HUMAN

NCBI Description

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq]

Uniprot Description

MKK3: a dual-specificity protein kinase of the STE7 family. Activates p38 MAP kinase by phosphorylating a Thr and a Tyr residue in the activation loop. Is activated by cytokines and environmental stress in vivo. A deletion and two point mutants found in colon cancer cell lines. Three splice-variant isoforms have been described.

Protein type: Kinase, protein; EC 2.7.12.2; Protein kinase, dual-specificity (non-receptor); Protein kinase, STE; STE group; STE7 family

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: nucleoplasm; membrane; cytosol

Molecular Function: MAP kinase kinase activity; protein binding; protein-tyrosine kinase activity; protein kinase binding; ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: peptidyl-tyrosine phosphorylation; activation of MAPK activity; MyD88-independent toll-like receptor signaling pathway; positive regulation of transcription, DNA-dependent; stress-activated MAPK cascade; pathogenesis; toll-like receptor 3 signaling pathway; signal transduction; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; positive regulation of protein kinase activity; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; inflammatory response; toll-like receptor 4 signaling pathway; regulation of cytokine biosynthetic process; cardiac muscle contraction