Western Blot (WB)
(Western blot analysis of extracts from HeLa, ME-180, 293 and K562 cells, using HR6A/HR6B Antibody.)
Rabbit anti-Human, Mouse HR6A Polyclonal Antibody
HR6A (HR6B)
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunocytochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
Purity
Affinity PurifiedPurified by Protein A and immunoaffinity chromatography.
Synonyms
HR6A; Polyclonal Antibody; HR6A (HR6B); Anti -HR6A (HR6B); anti-HR6A antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Detects endogenous levels of total HR6A and HR6B proteins.
Purity/Purification
Affinity Purified
Purified by Protein A and immunoaffinity chromatography.
Purified by Protein A and immunoaffinity chromatography.
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5,, 150 mM NaCl, 100ug/ml BSA and 50% glycerol.
Applicable Applications for anti-HR6A antibody
ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes
Suitable for use in ELISA, Immunofluorescence, Flow Cytometry, Western Blot and Immunocytochemistry.
Dilution: Flow Cytometry: 1:50
Western Blot: 1:1000
Immunocytochemistry (IF): 1:100
Dilution: Flow Cytometry: 1:50
Western Blot: 1:1000
Immunocytochemistry (IF): 1:100
Immunogen
Synthetic peptide (KLH-coupled) corresponding to amino acids at the amino-terminus of human HR6A.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis of extracts from HeLa, ME-180, 293 and K562 cells, using HR6A/HR6B Antibody.)
Western Blot (WB)
(Western blot analysis of extracts from HeLa, ME-180, 293 and K562 cells, using HR6A/HR6B Antibody.)
Immunofluorescence (IF)
(Immunofluorescent analysis of ME-180 cells, using HR6A/HR6B Antibody.)
Immunofluorescence (IF)
(Immunofluorescent analysis of ME-180 cells, using HR6A/HR6B Antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of untreated Jurkat cells, using HR6A/HR6B Antibody (blue) compared to a nonspecific negative control antibody (red).)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of untreated Jurkat cells, using HR6A/HR6B Antibody (blue) compared to a nonspecific negative control antibody (red).)
Related Product Information for anti-HR6A antibody
The ubiquitin-conjugating (UBC) enzymes HR6A and HR6B are the mammalian orthologues of the Saccharomyces cerevisiae Rad6 gene products (1). In S. cerevisiae, Rad6 facilitates cell cycle progression and ubiquitinates histone H2B (2,3). In vivo phosphorylation of HR6A Ser120 by cyclin-dependent kinases is thought to be important for the coordination and timing of ubiquitination events involved in cell cycle progression (4). In response to DNA damage, HR6A is known to interact physically with p53 and p14ARF, but knockout mice lacking HR6A or HR6B exhibit normal DNA damage responses (5,6). HR6B knockout males exhibit defective spermatogenesis, while HR6A knockout females fail to produce viable offspring (6).
Product Categories/Family for anti-HR6A antibody
Similar Products
Product Notes
The HR6A (Catalog #AAA618867) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HR6A (HR6B) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's HR6A can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS), Immunofluorescence (IF). Suitable for use in ELISA, Immunofluorescence, Flow Cytometry, Western Blot and Immunocytochemistry. Dilution: Flow Cytometry: 1:50 Western Blot: 1:1000 Immunocytochemistry (IF): 1:100. Researchers should empirically determine the suitability of the HR6A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HR6A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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