Rabbit anti-Mouse PCTAIRE 1 Polyclonal Antibody | anti-CDK16 antibody

PCTAIRE 1

Cat. Num. AAA618539

• Gene Names: CDK16; PCTK1; PCTAIRE; PCTAIRE1; PCTGAIRE
• Reactivity: Mouse
• Applications: Western Blot, Immunoprecipitation, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A and immunoaffinity chromatography.
• Synonyms: PCTAIRE 1; Polyclonal Antibody; Anti -PCTAIRE 1; anti-CDK16 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous levels of total PCTAIRE 1. Species Crossreactivity
• Purity/Purification: Affinity Purified; Purified by Protein A and immunoaffinity chromatography.
• Form/Format: Supplied in 10mM sodium HEPES, pH 7.5,, 150 mM NaCl, 0.1mg/ml BSA and 50% glycerol.

• Applicable Applications for anti-CDK16 antibody: Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Flow Cytometry and Immunoprecipitation.; Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.; Immunoprecipitation: 1:50; Flow Cytometry: 1:50
• Immunogen: Synthetic peptide derived from the sequence of human PCTAIRE 1 (KLH-coupled).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from CAD and Neuro2A cells, using MBS618539.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of untreated Neuro 2A cells, using MBS618539 (blue) compared to a nonspecific negative control antibody (red).)

Related Product Information for anti-CDK16 antibody

Three distinct PCTAIRE isoforms (PCTAIRE 1, PCTAIRE 2 and PCTAIRE 3) have been identified in humans and belong to the Cdk family of serine/threonine protein kinases. These proteins have a core kinase domain flanked by unique amino-and carboxy- terminal domains. Cdk proteins are known to regulate the cell cycle. All three PCTAIRE isoforms are abundantly expressed and catalytically active in post-mitotic brain, suggesting that they may function in processes other than cell division (1). PCTAIRE 1 is a cytoplasmic phosphoprotein whose kinase activity peaks in G2 and S phase (2). While one study indicates that noncovalent interactions with a regulatory component (such as a cyclin) are necessary for catalytic activity of PCTAIRE 1, others show that the monomeric protein is fully active (3). The Cdk5/p25 complex phosphorylates PCTAIRE 1 at Ser95, enhancing its kinase activity (4).

Product Categories/Family for anti-CDK16 antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 15990456
• NCBI GeneID: 5127
• UniProt Accession #: Q00536; A8K280; B7Z7C8; J3KN74; J3KQP7
• Molecular Weight: 55,716 Da
• NCBI Official Full Name: PCTAIRE protein kinase 1
• NCBI Official Synonym Full Names: cyclin-dependent kinase 16
• NCBI Official Symbol: CDK16
• NCBI Official Synonym Symbols: PCTK1; PCTAIRE; PCTAIRE1; PCTGAIRE
• NCBI Protein Information: cyclin-dependent kinase 16; PCTAIRE-motif protein kinase 1; cell division protein kinase 16; serine/threonine-protein kinase PCTAIRE-1
• UniProt Protein Name: Cyclin-dependent kinase 16
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: CDK16
• UniProt Synonym Gene Names: PCTAIRE1; PCTK1
• UniProt Entry Name: CDK16_HUMAN

NCBI Description

The protein encoded by this gene belongs to the cdc2/cdkx subfamily of the ser/thr family of protein kinases. It may play a role in signal transduction cascades in terminally differentiated cells; in exocytosis; and in transport of secretory cargo from the endoplasmic reticulum. This gene is thought to escape X inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2009]

Uniprot Description

CDK16: May play a role in signal transduction cascades in terminally differentiated cells. Found in a complex containing CABLES1, CDK17 and TDRD7. Interacts with YWHAH, YWHAQ and YWHAZ. Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Protein type: Protein kinase, CMGC; Motility/polarity/chemotaxis; Kinase, protein; Cell cycle regulation; EC 2.7.11.22; Protein kinase, Ser/Thr (non-receptor); CMGC group; CDK family; CDK/TAIRE subfamily; TAIRE subfamily

Chromosomal Location of Human Ortholog: Xp11

Cellular Component: microtubule cytoskeleton; synaptic vesicle; neuron projection; extrinsic to internal side of plasma membrane; transport vesicle; cytoplasm; plasma membrane; cell junction

Molecular Function: protein serine/threonine kinase activity; protein binding; cyclin-dependent protein kinase activity; ATP binding

Biological Process: growth hormone secretion; exocytosis; regulation of cell cycle; spermatogenesis; protein amino acid phosphorylation; neurite development