Western Blot (WB)
(Western blot analysis of extracts from ME-180 cells, using MBS625598.)
Rabbit anti-Human p63, alpha Polyclonal Antibody
p63, alpha (KET, p51, p40, AIS, p73L)
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunofluorescence
Purity
Affinity PurifiedPurified by Protein A and immunoaffinity chromatography.
Synonyms
p63; alpha; Polyclonal Antibody; alpha (KET; p51; p40; AIS; p73L); Anti -p63; anti-p63; alpha antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes endogenous levels of total human p63, alpha at ~75kD. Detects both full length TAp63, alpha and truncated delta, Np63, alpha, but not p63, beta or p63, gamma.
Purity/Purification
Affinity Purified
Purified by Protein A and immunoaffinity chromatography.
Purified by Protein A and immunoaffinity chromatography.
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Applicable Applications for anti-p63, alpha antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
Suitable for use in Western Blot, Immunoflourescence and Immunocytochemistry.
Dilution: Western Blot: 1:1000
Immunoflourescence (IF-IC): 1:100
Dilution: Western Blot: 1:1000
Immunoflourescence (IF-IC): 1:100
Immunogen
Synthetic peptide corresponding to residues within the C-terminus of human p63, alpha (KLH). Species Sequence Homology: Chicken.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of extracts from ME-180 cells, using MBS625598.)
Western Blot (WB)
(Western blot analysis of extracts from ME-180 cells, using MBS625598.)
Immunofluorescence (IF)
(Immunofluorescent analysis of ME-180 cells, using MBS625598.)
Immunofluorescence (IF)
(Immunofluorescent analysis of ME-180 cells, using MBS625598.)
Testing Data
Testing Data
Related Product Information for anti-p63, alpha antibody
The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis (1). In addition to p53, mammalian cells contain two p53 family members, p63 and p73, which are similar to p53 in both structure and function (2). While p63 can induce p53-responsive genes and apoptosis, mutation of p63 rarely results in tumors (2). Amplification of the p63 gene is frequently observed in squamous cell carcinomas of the lung, head and neck (2,3). The p63 gene contains an alternative transcription intiation site, which yields a 40kD delta, Np63 lacking the transactivation domain, and alternative splicing at the carboxy-terminus yields the alpha, beta and gamma isoforms (3,4).
Product Categories/Family for anti-p63, alpha antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
p63
Similar Products
Product Notes
The p63, alpha (Catalog #AAA625598) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The p63, alpha (KET, p51, p40, AIS, p73L) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's p63, alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). Suitable for use in Western Blot, Immunoflourescence and Immunocytochemistry. Dilution: Western Blot: 1:1000 Immunoflourescence (IF-IC): 1:100. Researchers should empirically determine the suitability of the p63, alpha for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p63, alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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