Rabbit anti-Human Cyclin E Polyclonal Antibody

Cyclin E, phosphorylated (Thr62)

Cat. Num. AAA630514

• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Protein A and peptide affinity chromatography
• Synonyms: Cyclin E; Polyclonal Antibody; phosphorylated (Thr62); Anti -Cyclin E; anti-Cyclin E antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Detects endogenous levels of cyclin E only when phosphorylated at threonine 62 (cyclin E1 isoform 2) or threonine 77 (cyclin E1 isoform 1). Species Crossreactivity: Human
• Purity/Purification: Affinity Purified; Protein A and peptide affinity chromatography
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM NaCl, 100ug/ml BSA, 50% glycerol.

• Applicable Applications for anti-Cyclin E antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Flow Cytometry, Western Blotting, Immunoprecipitation, Immunohistochemistry.; Dilution: Flow Cytometry: 1:25; Western Blotting: 1:1000; Immunohistochemistry: (Paraffin) 1:100 Citrate/TBST; Immunoprecipitation: 1:100
• Immunogen: Synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Thr62 of human cyclin E.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot. Store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from 293 cells, treated with 10mM LiCl for the indicated times, using MBS630514 Cyclin E Phosphorylated (Thr62) (upper) or a cyclin E (lower).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using MBS630514 Cyclin E Phosphorylated (Thr62))

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of untreated Jurkat cells, using MBS630514 Cyclin E Phosphorylated (Thr62) versus propidium iodide (DNA content). The boxed population indicates phospho-Cyclin E (Thr62)-positive cells.)

Related Product Information for anti-Cyclin E antibody

Cyclin E1 and cyclin E2 can associate with and activate cdk2 (1). Upon DNA damage, upregulation/ activation of the cdk inhibitors p21Waf1/Cip1 and p27Kip1 prevents cyclin E/cdk2 activation, resulting in G1/S arrest. However, when conditions are favorable for cell cycle progression, cyclin D/cdk4/6 phosphorylates Rb and is thought to reduce the activity of p21Waf1/Cip1 and p27Kip1, allowing subsequent activation of cyclin E/cdk2 (1,2). Cyclin E/cdk2 then further phosphorylates Rb to allow progression into S-phase, where cyclin

Product Categories/Family for anti-Cyclin E antibody

Antibodies; Abs to Cyclin

NCBI and Uniprot Product Information

• NCBI GI #: 1117990
• NCBI Official Full Name: cyclin E

Uniprot Description

CCNE1: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. Accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Two alternatively spliced isoforms have been described.

Protein type: Nuclear receptor co-regulator; Cell cycle regulation; Activator

Chromosomal Location of Human Ortholog: 19q12

Cellular Component: nucleoplasm; cyclin-dependent protein kinase holoenzyme complex; nucleus; cytosol

Molecular Function: protein binding; androgen receptor binding; transcription coactivator activity; kinase activity; cyclin-dependent protein kinase regulator activity; protein kinase binding

Biological Process: G1/S-specific transcription in mitotic cell cycle; Wnt receptor signaling pathway; DNA replication initiation; cell division; regulation of cell cycle; positive regulation of transcription, DNA-dependent; androgen receptor signaling pathway; mitotic cell cycle; regulation of protein kinase activity; protein amino acid phosphorylation; G1/S transition of mitotic cell cycle