Goat CD99-L2 Polyclonal Antibody | anti-CD99 antibody

CD99-L2 (CD99 antigen-like 2)

Cat. Num. AAA618457

• Gene Names: CD99; MIC2; HBA71; MIC2X; MIC2Y; MSK5X
• Applications: ELISA, Western Blot, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: CD99-L2; Polyclonal Antibody; CD99-L2 (CD99 antigen-like 2); Anti -CD99-L2 (CD99 antigen-like 2); anti-CD99 antibody

• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse CD99-L2, extracellular domain. Shows approximately 10% cross-reactivity with rhCD99-L2 and less than 5% cross-reactivity with rmCD99.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as lyophilized from a 0.2um sterile-filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS. If 0.5ml of PBS is used, the antibody concentration will be 0.2mg/ml.

• Applicable Applications for anti-CD99 antibody: ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in ELISA, Western Blot, Flow Cytometry.; Dilution: Western Blot: 0.1-0.2ug/ml with the appropriate secondary reagents to detect mouse CD99-L2. The detection limit for rmCD99-L2 is approximately 2ng/lane under non-reducing and reducing; conditions.; Flow Cytometry: Dilute this antibody to 25ug/ml and add 10ul of the diluted solution to 1-2.5x10e5 cells in a total reaction volume not exceeding 200ul, using bEnd.3 cells (Schenkel AR, et al., (2007) Cell Commun. Adhes. 14:227). The binding of unlabeled antibodies may be visualized by adding a secondary developing reagent such as anti-goat IgG conjugated to a fluorochrome. Between steps, wash cells in Flow Cytometry Staining Buffer.; ELISA: 0.5-1ug/ml with the appropriate secondary reagents to detect mouse CD99-L2. The detection limit for rmCD99-L2 is approximately 0.2ng/well.
• Immunogen: NS0-derived rmCD99-L2 extracellular domain.
• Endotoxin Level: 0.25 EU per 1ug of the antibody as determined by the LAL method.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-CD99 antibody

CD99 antigen-like 2 (CD99-L2) is a 45kD type I transmembrane glycoprotein in the CD99 family of molecules (1-3). Mouse CD99-L2 cDNA encodes a 214 amino acid (aa) precursor with a 23 aa predicted signal sequence, a 116 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 54 aa cytoplasmic region (4). The ECD contains no N-linked glycosylation sites, but O-linked glycosylation is likely (1, 2). A long isoform with a 23 aa insert after aa 43 within the ECD is expressed in human, and probably in mouse (2, 5). Both long and short isoforms may have minor variants missing portions of the N-terminus of the mature protein (6). The ECD of mouse CD99-L2 short isoform shares 85%, 72% and 66% aa identity with rat, human, and bovine CD99-L2, respectively (3, 5). The mouse CD99 and CD99-L2 ECDs share only 31% aa identity, but both contain three conserved acidic motifs and are thought to originate from the same ancestral gene (1, 2). The nearly ubiquitous expression of CD99-L2 is similar to that of CD99. Mouse CD99-L2 shows highest in situ hybridization signals in neurons, cortical thymocytes, ganglia, ovarian granulosa cells, testis, and kidney, and detectable protein levels in lung, thymocytes, mouse leukocytes and vascular endothelial cells (2, 7). CD99-L2 expression on endothelial cells is reported to mediate cell aggregation and neutrophil or monocyte extravasation to inflamed tissue in vivo, while CD99 mediates lymphocyte extravasation as well (3, 7).

Product Categories/Family for anti-CD99 antibody

Antibodies; Abs to CD Markers

NCBI and Uniprot Product Information

• NCBI GI #: 171543879
• NCBI GeneID: 4267
• NCBI Accession #: NP_001116370.1
• NCBI GenBank Nucleotide #: NM_001122898.1
• UniProt Accession #: P14209; O00518; Q6ICV7
• Molecular Weight: 18,848 Da
• NCBI Official Full Name: CD99 antigen isoform b
• NCBI Official Synonym Full Names: CD99 molecule
• NCBI Official Symbol: CD99
• NCBI Official Synonym Symbols: MIC2; HBA71; MIC2X; MIC2Y; MSK5X
• NCBI Protein Information: CD99 antigen; E2 antigen; OTTHUMP00000022840; OTTHUMP00000022842; OTTHUMP00000022843; OTTHUMP00000022844; surface antigen MIC2; T-cell surface glycoprotein E2; MIC2 (monoclonal antibody 12E7); antigen identified by monoclonal 12E7, Y homolog; antigen identified by monoclonal antibodies 12E7, F21 and O13
• UniProt Protein Name: CD99 antigen
• Protein Family: CD99 antigen
• UniProt Gene Name: CD99
• UniProt Synonym Gene Names: MIC2; MIC2X; MIC2Y
• UniProt Entry Name: CD99_HUMAN

NCBI Description

The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of CD99. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Uniprot Description

CD99: Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes. Belongs to the CD99 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xp22.32 and Yp11.3

Cellular Component: focal adhesion; integral to plasma membrane; cytoplasm; plasma membrane

Biological Process: cell adhesion