CD99/MIC2 recombinant protein

Recombinant Human CD99/MIC2 (C-6His)

Cat. Num. AAA2569059

• Gene Names: CD99; MIC2; HBA71; MIC2X; MIC2Y; MSK5X
• Purity: >90% as determined by reducing SDS-PAGE
• Synonyms: CD99/MIC2; Recombinant Human CD99/MIC2 (C-6His); CD99 Antigen; 12E7; E2 Antigen; Protein MIC2; T-Cell Surface Glycoprotein E2; CD99; MIC2; MIC2X; MIC2Y; CD99/MIC2 recombinant protein

• Host: Human Cells
• Purity/Purification: >90% as determined by reducing SDS-PAGE
• Form/Format: Lyophilized from a 0.2um filtered solution of 20mM PB, 150mM NaCl, pH7.4.
• Sequence: Asp23-Asp122

• Species: Human
• Tag: C-6His
• Endotoxin: <1.0EU per ug as determined by the LAL method.
• Protein Construction: Recombinant Human CD99 is produced by our Mammalian expression system and the target gene encoding Asp23-Asp122 is expressed with a 6His tag at the C-terminus.
• Preparation and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.; This product is provided as lyophilized powder which is shipped with ice packs.

SDS-Page

Related Product Information for CD99/MIC2 recombinant protein

CD99 is a type I transmembrane glycoprotein and the founding member of the CD99 family of molecules. The extracellular domain of CD99 contains no identifiable motifs, its cytoplasmic region, although short, does have signal transduction capability. Cells known to express CD99 include fibroblasts, neutrophils, T cells, double positive thymocytes, CD34+ stem cells, monocytes and endothelial cells. Two types of CD99 isoforms have been classified. Native human CD99 is referred to as the long, or type I isoform. The best studied type II isoform shows an Asp-Gly substitution for the C terminal 27 amino acids. The type I and II isoforms have distinctive signal transduction pathways (FAKsrc for type I PI3K plus srcERK1/2 for type II), and mediate clearly different biological outcomes. Homophilic interaction between CD99 on the neutrophil and CD99 on the endothelial cell regulates the transendothelial migration of neutrophils during inflammation. Human CD99 has 48% aa sequence identity to mouse CD99.

Product Categories/Family for CD99/MIC2 recombinant protein

Signal Transduction; Cancer; immunology

NCBI and Uniprot Product Information

• NCBI GI #: 4505183
• NCBI GeneID: 4267
• NCBI Accession #: NP_002405.1
• NCBI GenBank Nucleotide #: NM_002414.3
• UniProt Accession #: P14209; O00518; Q6ICV7; A6NIW1
• Molecular Weight: 18,848 Da
• NCBI Official Full Name: CD99 antigen isoform a
• NCBI Official Synonym Full Names: CD99 molecule
• NCBI Official Symbol: CD99
• NCBI Official Synonym Symbols: MIC2; HBA71; MIC2X; MIC2Y; MSK5X
• NCBI Protein Information: CD99 antigen; E2 antigen; surface antigen MIC2; T-cell surface glycoprotein E2; MIC2 (monoclonal antibody 12E7); antigen identified by monoclonal 12E7, Y homolog; antigen identified by monoclonal antibodies 12E7, F21 and O13
• UniProt Protein Name: CD99 antigen
• UniProt Gene Name: CD99
• UniProt Synonym Gene Names: MIC2; MIC2X; MIC2Y
• UniProt Entry Name: CD99_HUMAN

NCBI Description

The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of CD99. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2013]

Uniprot Description

CD99: Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes. Belongs to the CD99 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xp22.32 and Yp11.3

Cellular Component: focal adhesion; integral to plasma membrane; cytoplasm; plasma membrane

Biological Process: cell adhesion