Western Blot (WB)
(Western Blot analysis of cell lysates from various cell lines using MBS616701.)
Rabbit LSD1 Polyclonal Antibody
LSD1 (Lysine-Specific Histone Demethylase, Amine Oxidase Flavin-containing Domain Protein 2, AOF2)
Reactivity
Monkey, Mouse, Rat
Applications
ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
Purity
Affinity PurifiedAffinity Purified.
Synonyms
LSD1; Polyclonal Antibody; LSD1 (Lysine-Specific Histone Demethylase; Amine Oxidase Flavin-containing Domain Protein 2; AOF2); Anti -LSD1 (Lysine-Specific Histone Demethylase; anti-LSD1 antibody
Host
Rabbit
Reactivity
Monkey, Mouse, Rat
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of total human LSD1 protein. Species crossreactivity: mouse, rat and monkey.
Purity/Purification
Affinity Purified
Affinity Purified.
Affinity Purified.
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA and 50% glycerol.
Applicable Applications for anti-LSD1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes
Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry.
Dilution: Western blot 1:1000
Immunoprecipitation 1:25
Immunohistochemistry (Paraffin) 1:200
IHC Protocol: Citrate/TBST
Immunofluorescence (IF-IC) 1:100
Flow Cytometry 1:100
Dilution: Western blot 1:1000
Immunoprecipitation 1:25
Immunohistochemistry (Paraffin) 1:200
IHC Protocol: Citrate/TBST
Immunofluorescence (IF-IC) 1:100
Flow Cytometry 1:100
Immunogen
Synthetic peptide (KLH-coupled) corresponding to the carboxy terminus of human LSD1 protein.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western Blot analysis of cell lysates from various cell lines using MBS616701.)
Western Blot (WB)
(Western Blot analysis of cell lysates from various cell lines using MBS616701.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma using MBS616701)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma using MBS616701)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma using MBS616701. Preincubated with a control peptide (left) or antigen-specific peptide (right).)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma using MBS616701. Preincubated with a control peptide (left) or antigen-specific peptide (right).)
Related Product Information for anti-LSD1 antibody
Lysine-specific demethylase 1 (LSD1; also known as AOF2 and BHC110) is a nuclear homolog of amine oxidases and functions as a histone demethylase and transcription cofactor (1). Gene activation and repression is specifically regulated by the methylation state of distinct lysine residues in histone proteins. For example, methylation of histone H3 Lys4 facilitates transcriptional activation by coordinating the recruitment of BPTF, a component of the NURF chromatin remodeling complex, and WDR5, a component of multiple histone methyltransferase complexes (2,3). In contrast, methylation of histone H3 (Lys9) facilitates transcriptional repression by recruiting HP1 (4,5). LSD1 is a component of the CoREST transcriptional co-repressor complex, which contains CoREST, CtBP, HDAC1 and HDAC2. As part of this complex, LSD1 demethylates mono-methyl and di-methyl histone H3 Lys4 via an FADdependent oxidation reaction, and facilitates the repression of neuronal-specific genes in non-neuronal cells (1,6,7). In contrast, when LSD1 is associated with androgen-receptor in human prostate cells, it demethylates mono-methyl and di-methyl histone H3 Lys9 and facilitates androgen-receptordependent transcriptional activation (8). Therefore, LSD1 can function as a co-repressor or co-activator, depending on gene context. LSD1 activity is inhibited by the amine oxidase inhibitors pargyline, deprenyl, clorgyline and tranylcypromine (8).
Product Categories/Family for anti-LSD1 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The LSD1 (Catalog #AAA616701) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LSD1 (Lysine-Specific Histone Demethylase, Amine Oxidase Flavin-containing Domain Protein 2, AOF2) reacts with Monkey, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's LSD1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF). Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Dilution: Western blot 1:1000 Immunoprecipitation 1:25 Immunohistochemistry (Paraffin) 1:200 IHC Protocol: Citrate/TBST Immunofluorescence (IF-IC) 1:100 Flow Cytometry 1:100. Researchers should empirically determine the suitability of the LSD1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LSD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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