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Western Blot (WB)
(Western Blot analysis in 293 cell lysates (2ug/lane) using MBS646290. Lane 1: Nontransfected Lane 2: Transiently transfected with the AOF2 gene.)
Rabbit anti-Human LSD1 Polyclonal Antibody | anti-kdm1a antibody
LSD1, CT (KDM1A, AOF2, KDM1, KIAA0601, LSD1, Lysine-specific histone demethylase 1A, BRAF35-HDAC complex protein BHC110, Flavin-containing amine oxidase domain-containing protein 2)
Gene Names
kdm1a; aof2; kdm1; wu:fb82e06; wu:fc51a02; wu:fj36a05; wu:fj66b06
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
PurifiedPurified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Synonyms
LSD1; Polyclonal Antibody; CT (KDM1A; AOF2; KDM1; KIAA0601; Lysine-specific histone demethylase 1A; BRAF35-HDAC complex protein BHC110; Flavin-containing amine oxidase domain-containing protein 2); Anti -LSD1; anti-kdm1a antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Purified
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-kdm1a antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Dilution: ELISA: 1:1,000
Western BLot 1:100-500
Immunohistochemistry: 1:50-100
Dilution: ELISA: 1:1,000
Western BLot 1:100-500
Immunohistochemistry: 1:50-100
Immunogen
LSD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human LSD1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis in 293 cell lysates (2ug/lane) using MBS646290. Lane 1: Nontransfected Lane 2: Transiently transfected with the AOF2 gene.)
Western Blot (WB)
(Western Blot analysis in 293 cell lysates (2ug/lane) using MBS646290. Lane 1: Nontransfected Lane 2: Transiently transfected with the AOF2 gene.)
Western Blot (WB)
(Western Blot of nuclear extracts of control and PhIP-treated HMEC. Proteins were transferred onto polyvinylidene difluoride and blotted with MBS646290. Nuclear LSD1 protein levels increased in carcinogen-treated HMEC compared with control HMEC.)
Western Blot (WB)
(Western Blot of nuclear extracts of control and PhIP-treated HMEC. Proteins were transferred onto polyvinylidene difluoride and blotted with MBS646290. Nuclear LSD1 protein levels increased in carcinogen-treated HMEC compared with control HMEC.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of formalin-fixed and paraffin-embedded human cancer tissue using MBS646290 followed by peroxidase-conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS646290 for immunohistochemistry)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of formalin-fixed and paraffin-embedded human cancer tissue using MBS646290 followed by peroxidase-conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS646290 for immunohistochemistry)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of 293 cells using MBS646290 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of 293 cells using MBS646290 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).)
Related Product Information for anti-kdm1a antibody
LSD1 is a histone demethylase that specifically demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. LSD1 contains a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. It acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. LSD1 demethylates both mono- and tri-methylted 'Lys-4' of histone H3. This protein may play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 'Lys-4' on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. It may also demethylate 'Lys-9' of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes.
Product Categories/Family for anti-kdm1a antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
lysine-specific histone demethylase 1A
NCBI Official Synonym Full Names
lysine (K)-specific demethylase 1a
NCBI Official Symbol
kdm1a
NCBI Official Synonym Symbols
aof2; kdm1; wu:fb82e06; wu:fc51a02; wu:fj36a05; wu:fj66b06
NCBI Protein Information
lysine-specific histone demethylase 1A; lsd1; lysine (K)-specific demethylase 1; amine oxidase (flavin containing) domain 2
Protein Family
Similar Products
Product Notes
The kdm1a (Catalog #AAA646290) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LSD1, CT (KDM1A, AOF2, KDM1, KIAA0601, LSD1, Lysine-specific histone demethylase 1A, BRAF35-HDAC complex protein BHC110, Flavin-containing amine oxidase domain-containing protein 2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LSD1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot, Immunohistochemistry, ELISA Dilution: ELISA: 1:1,000 Western BLot 1:100-500 Immunohistochemistry: 1:50-100. Researchers should empirically determine the suitability of the kdm1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LSD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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