Rabbit LSD1 Polyclonal Antibody | anti-LSD1 antibody

LSD1 Antibody

Cat. Num. AAA9409372

• Gene Names: KDM1A; AOF2; KDM1; LSD1; BHC110
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity chromatography purified via peptide column
• Synonyms: LSD1; Polyclonal Antibody; LSD1 Antibody; Lysine-specific histone demethylase; amine oxidase flavin containing domain protein 2; AOF2; anti-LSD1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 876

• Applicable Applications for anti-LSD1 antibody: ELISA (EIA), Western Blot (WB)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a 17 amino acid peptide from near the amino terminus of human LSD1.
• Target Name: LSD1
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of LSD1 in P815 cell lysate with LSD1 antibody at (A) 1 and (B) 2 ug/mL.)

Related Product Information for anti-LSD1 antibody

Histone modifications mediate changes in gene expression by altering chromatin structure or by serving as a platform to recruit other proteins. LSD1 is a recently discovered amine oxidase that catalyzes the lysine-specific demethylation of histone proteins via an FAD-dependent oxidative reaction. Methylation on histone H3-K9 is thought to play an important role in heterochromatin formation, while methylation on arginine and some lysine residues (such as H3-K4) is associated with active transcription. LSD1 associates with various proteins, including HDAC1/2, CoREST, and BHC80, that act to regulate LSD1 activity in vivo, and in a histone H3-K4-specific methylase complex that is involved in transcriptional regulation. Experiments have shown that CoREST, a SANT domain-containing corepressor acts to enhance LSD1 activity, while BHC80, a PHD domain-containing protein, inhibits CoREST/LSD1 activity in vitro. LSD1-mediated histone demethylation thus may have significant effects on gene expression.

Product Categories/Family for anti-LSD1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 58761544
• NCBI GeneID: 23028
• NCBI Accession #: NP_001009999
• NCBI GenBank Nucleotide #: NM_001009999.2
• Molecular Weight: 95,155 Da
• NCBI Official Full Name: lysine-specific histone demethylase 1A isoform a
• NCBI Official Synonym Full Names: lysine (K)-specific demethylase 1A
• NCBI Official Symbol: KDM1A
• NCBI Official Synonym Symbols: AOF2; KDM1; LSD1; BHC110
• NCBI Protein Information: lysine-specific histone demethylase 1A
• UniProt Protein Name: Lysine-specific histone demethylase 1A
• Protein Family: Lysine-specific histone demethylase
• UniProt Gene Name: KDM1A
• UniProt Synonym Gene Names: AOF2; KDM1; KIAA0601; LSD1
• UniProt Entry Name: KDM1A_HUMAN

NCBI Description

This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]

Uniprot Description

LSD1: Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr- 6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Component of a RCOR/GFI/KDM1A/HDAC complex. Interacts directly with GFI1 and GFI1B. Component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B, KDM1A, RCOR1 and PHF21A. The BHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. In the complex, RCOR1/CoREST strongly enhances the demethylase activity and protects it from the proteasome while PHF21A/BHC80 inhibits the demethylase activity. Interacts with the androgen receptor (AR). Interacts with ASXL1. Ubiquitously expressed. Belongs to the flavin monoamine oxidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; Nuclear receptor co-regulator; EC 1.-.-.-; Demethylase; Oxidoreductase

Chromosomal Location of Human Ortholog: 1p36.12

Cellular Component: nucleoplasm; transcription factor complex; nuclear chromatin; nucleus

Molecular Function: FAD binding; p53 binding; oxidoreductase activity; transcription factor binding; histone demethylase activity (H3-K9 specific); histone demethylase activity (H3-K4 specific); protein binding; MRF binding; enzyme binding; androgen receptor binding; ligand-dependent nuclear receptor transcription coactivator activity; histone demethylase activity; chromatin binding; transcription factor activity; demethylase activity

Biological Process: negative regulation of histone H3-K9 methylation; establishment and/or maintenance of chromatin architecture; granulocyte differentiation; transcription, DNA-dependent; in utero embryonic development; negative regulation of transcription factor activity; positive regulation of erythrocyte differentiation; muscle cell development; negative regulation of histone H3-K4 methylation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of DNA binding; protein amino acid demethylation; regulation of transcription from RNA polymerase II promoter; cell proliferation; negative regulation of DNA damage response, signal transduction by p53 class mediator; histone H3-K9 demethylation; pituitary gland development; positive regulation of hormone biosynthetic process; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of megakaryocyte differentiation; negative regulation of transcription, DNA-dependent; blood coagulation; negative regulation of protein binding